| Abstract: | Second harmonic generation (SHG) microscopy is employed to study changes in crystallineorganization due to altered gene expression and hydration in barley starch granules. SHGintensity and susceptibility ratio values (R’SHG) areobtained using reduced Stokes-Mueller polarimetric microscopy. The maximumR’SHG values occur at moderate moisture indicating thenarrowest orientation distribution of nonlinear dipoles from the cylindrical axis of glucanhelices. The maximum SHG intensity occurs at the highest moisture and amylopectin content.These results support the hypothesis that SHG is caused by ordered hydrogen and hydroxyl bondnetworks which increase with hydration of starch granules.OCIS codes: (170.3880) Medical and biological imaging, (170.4580) Optical diagnostics for medicine |
