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In vitro proliferation of primary human head and neck squamous cell carcinomas evaluated by flow cytometry |
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| Authors: | J T Bijman D J Wagener J M Wessels D Elprana P van den Broek |
| Affiliation: | 1. Division of Electronics and Electrical Engineering, Dongguk University-Seoul, Seoul, South Korea;2. Department of Chemical Engineering, Hanyang University, Seongdong-gu, Seoul, South Korea;3. Department of Physics, National Engineering College, K.R. Nagar, Kovilpatti, Tamil Nadu, India;4. Research Centre for Carbon Solutions, Heriot-Watt University, Edinburgh, United Kingdom;5. Department of Mechanical Engineering, School of Mechanical and Manufacturing Engineering, National University of Sciences and Technology (NUST), Islamabad, Pakistan;6. Department of Hydrogen and Fuel Cell Technology, Hanyang University, Seoul, South Korea;1. Institute for Sustainable Agriculture, CSIC, P.O. Box 4084, 14080-Córdoba, Spain;2. Department of Computer Science and Numerical Analysis, University of Córdoba, Rabanales Campus, C2 building, 14071-Córdoba, Spain;3. Department of Mathematics and Engineering, Universidad Loyola Andalucía, third building, 14004-Córdoba, Spain |
| Abstract: | In vitro proliferation of primary human head and neck squamous cell carcinomas was investigated using single cell suspensions and tissue explants of primary specimens and xenografts from 20 tumor specimens. The evaluations of the cells emerging in culture were performed with flow cytometry. Epithelial-like cells proliferated in serum-free medium, while no fibroblast-like cells were observed in culture. The epithelial-like cells could be subcultured several passages before senescence occurred. Conditioned medium or serum supplementation was necessary for a sustained outgrowth of malignant squamous cells as documented by flow cytometry. From a tumor line established in nude mice slowly proliferating tumor cells emerged. After 4-5 months in culture tumor cells seemed to be adapted to the culture conditions used. This resulted in a more consistent tumor cell proliferation. Early passage cultures from primary human head and neck squamous cell carcinomas are clearly difficult to obtain either from primary human specimens or from tumor lines established in nude mice. |
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