| 抗人白细胞间素-2 McAb亲和力排列及亲和指数的测定 |
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| 引用本文： | 王小宁,高基民,马筠,李文简,马贤凯,汪美先. 抗人白细胞间素-2 McAb亲和力排列及亲和指数的测定[J]. 细胞与分子免疫学杂志, 1990, 0(1) |
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| 作者姓名： | 王小宁 高基民 马筠 李文简 马贤凯 汪美先 |
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| 作者单位： | 第一军医大学免疫学教研室，第一军医大学免疫学教研室，北京第二传染病医院肝炎研究所，第一军医大学免疫学教研室，军事医学科学院基础所分子遗传室，第四军医大学微生物学教研室 广州 510515，广州 510515，广州 510515，北京 100850，西安 710032 |
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| 摘 要： | 利用蛋白浓度校正的ELISA稀释曲线快速确定了抗人白细胞间素-2(IL-2)单克隆抗体(McAb)的亲和力排列。该排列与用竞争ELISA测定的亲和常数(K_D值)相一致。为了更实际地确定McAb的用途,又用十二烷基磺酸钠(SDS)等解离因素,建立了反映 McAb在实际应用时亲和力变化强度的吸附-解离ELISA,测定McAb的亲和指数(ID)。根据亲和力排列和亲和指数可更准确地取舍McAb,供检测或亲和色谱使用。
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| 关 键 词： | 白细胞间素类 抗体 单克隆 亲和力 色谱法 亲和 |
The Determination of Affinity Indexes and Affinity Ranking of Monoclonal Antibodies against Interleukin-2 |
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| Wang Xiaoning,Gao Jimin,Ma Yun,Li Wenjian,Ma Xiankai,Wang Meixian. The Determination of Affinity Indexes and Affinity Ranking of Monoclonal Antibodies against Interleukin-2[J]. Chinese journal of cellular and molecular immunology, 1990, 0(1) |
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| Authors: | Wang Xiaoning Gao Jimin Ma Yun Li Wenjian Ma Xiankai Wang Meixian |
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| Abstract: | The affinities of the monoclonal antibodies (McAb) against human interleu-kin-2 were ranked easily by their protein concentration-adjusted ELISA dilution curves. The ranking was consistent with the'ranking of their KD values measured by a competitive ELISA method. In order to reflect the virtual affinity changes of the McAb in practical uses, an absorption elution sandwich ELISA assay was established to obtain the affinity indexes, which were the ratio of absorbance value produced by one McAb in normal antigen-absorbing condition to that in elution condition ( with low concentration of detergents or low pH ) . By affinity ranking and affinity indexes, we can choose more proper antibodies used either for detection assay or for affinity chromatography. |
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| Keywords: | interleukins antibodies monoclonal affinity chromatography affinit |
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