Participation of methylcobalamin inin vitro DNA methylation |
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| Authors: | R I Klyasheva E V Sharkova I I Nikol'skaya E V Belozerova A M Yurkevich |
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| Institution: | (1) Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, Moscow;(2) Vitamin Research-and-Manufacturing Association, Moscow;(3) Department of Medicine, I. N. Ul'yanov Chuvash State University, Cheboksary |
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| Abstract: | Two DNA-methylases from nuclei of rat liver are compared for their ability to methylate thymic DNA in the presence of methyl
group donors, radiolabeled3H-S-adenosyl-L-methionine and methylcobalamin. DNA-methylase with an isoelectric point of 5.2 is found, which uses both donors
of methyl groups although its activity is more expressed with3H-S-adenosyl-L-methionine.3H-S-adenosyl-L-methionine and methylcobalamin presumably interact with different sites of the enzyme.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N
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6, pp. 616–618, June, 1995
Presented by T. T. Berezov, Member of the Russian Academy of Medical Sciences |
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| Keywords: | DNA DNA-methylase 5-methylcytosine S-adenosyl-L-methionine methylcobalamin vitamin B 12 donors of methyl groups |
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