补充微量营养素减轻应激损伤的实验研究

目的　观察复合微量营养素对大鼠应激损伤的预防作用。方法　按低、中、高 3种剂量给实验大鼠补充含有维生素、氨基酸和矿物元素的复合营养素 ,以间断足底电击建立动物应激模型 ,检测其旷场行为、应激反应、应激蛋白及抗氧化功能的变化。结果　足底电击诱导的应激反应引起实验大鼠行为异常改变 ,血浆皮质醇浓度升高 ,脑组织儿茶酚胺含量减少。应激动物肝脏和脑组织中的金属硫蛋白含量出现不同程度的变化 ,同时血清总抗氧化能力降低而肝组织脂质过氧化物水平增加。补充 6周微量营养素的应激大鼠行为异常得到逆转 ,上述血液和组织的多数生化指标也出现明显改善。结论　补充复合微量营养素有利于提高实验动物的应激适应能力 ,减轻机体的应激反应损伤

Supplementation with micronutrients attenuating stress injuries in rats

Objective To observe the protective effects of compound micronutrients (CMN)on stress induced injury of the rats. Methods The experimental rats were fed with diet added micronutrients in small, medium and large dose respectively. The stress animal model was established by feet electric shock. The behaviors in open field test, hormons, metallothionein (MT) and the antioxidant ability of the rats were detected. Results Compared with control group, the abnormal behaviors in open field test, increased cortisol level in serum, decreased epinephrine and norepinephrine contents in brain were observed in stress rats. Also stress induced significantly changes of MT levels in liver and brain, and declined antioxidant ability in serum and enhanced lipid peroxidation in liver of the animals. The most indicators mentioned above were obviously improved by supplementation with CMN for 6 weeks. Conclusion Supplementation with CMN was beneficial to improve the stress adaptating ability and to attenuate the strss induced injuries in rats.