不同剂量附子水煎液对慢性心力衰竭小鼠的干预作用

目的：探讨不同剂量附子水煎液对慢性心力衰竭（CHF）小鼠的影响。方法：将53只C57/6J小鼠雄性随机分为对照组、模型组、附子水煎液低剂量组、附子水煎液中剂量组、附子水煎液高剂量组，用盐酸阿霉素（Dox）腹腔注射诱导CHF小鼠模型，药物干预给予不同浓度（0.2、0.6、2 mg/kg）的附子水煎液进行灌胃治疗，1次/d，连续干预30 d，统计小鼠生存率和一般指标，评价小鼠心脏功能，观察心肌组织病理形态学的变化，检测血清中脑钠肽（BNP）和心肌肌钙蛋白I（cTnI）的含量，检测心钠尿肽（ANP）和BNP的mRNA表达水平，检测活化的天冬氨酸特异性半胱氨酸蛋白酶（Cleaved-caspase3）和凋亡蛋白Bax（Bax）的表达情况。结果：与模型组比较，不同剂量附子水煎液组小鼠心脏功能均得到改善（P<0.05），且高剂量附子水煎剂组改善最为显著，具体表现为心肌组织横截面积增大，血清BNP和cTnI的含量降低（P<0.05），BNP的mRNA表达水平和Cleaved-caspase3及Bax蛋白表达水平明显降低（P<0.05）。结论：附子水煎液能剂量依赖性地抑制心肌细胞凋亡，减轻小鼠心功能及其病理结构的改变，进而改善Dox诱导的CHF。

Interventional Effects of Different Doses of Aconite Decoction on Mice with Chronic Heart Failure

To investigate the effects of different doses of aconite decoction on mice with chronic heart failure(CHF).Methods:Fifty-three healthy male C57/6J mice were randomly divided into a control group,a model group,and low,middle,and high-dose aconite decoction groups.CHF mouse models were induced by intraperitoneal injection of doxorubicin(Dox).The mice were treated with aconite decoction at different concentrations(0.2,0.6,2 mg/kg) via intragastric administration once daily for 30 consecutive days.The survival rate and general indexes were recorded,cardiac function was evaluated,and histopathological changes in myocardial tissue were observed.Serum levels of brain natriuretic peptide(BNP) and cardiac troponin I(cTnI) were detected.The mRNA expression levels of atrial natriuretic peptide(ANP) and BNP were detected.Additionally,expression levels of activated aspartate-specific caspase(cleaved-caspase-3) and apoptotic protein Bax(Bcl2-associated X protein,Bax) were detected.Results:Compared with the model group,the cardiac function of mice in all aconite decoction groups improved(P<0.05),with the most significant improvement observed in the high-dose aconite decoction group,as indicated by an increase in myocardial tissue cross-section.Serum BNP and cTnI levels decreased(P<0.05).The mRNA expression level of BNP and the protein expression levels of cleaved-caspase-3 and Bax significantly decreased(P<0.05).Conclusion:Aconite decoction inhibits myocardial cell apoptosis in a dose-dependent manner,reduces the changes in cardiac function and pathological structure in mice,and thereby improves Dox-induced CHF.