3-羟基苯甲醛抑制兔自体移植静脉桥内膜增生的研究

目的:研究3-羟基苯甲醛(3-HBA)对兔搭桥后静脉桥内膜增生的影响.方法:24只新西兰兔计算机随机均分实验组和对照组,建立颈外静脉移植到颈总动脉实验模型,实验组动物予以耳缘静脉注射3-HBA 100mg/(kg﹒d),对照组动物予以同剂量生理盐水.于术后的2周、4周采集各组移植血管,计算移植血管桥内膜厚度,Western Blot检测AKT蛋白活化情况.人大隐静脉平滑肌细胞进行体外培养,不同浓度3-HBA作用于平滑肌细胞,MTT法检测细胞增殖,Transwell小室法检测细胞迁移.结果:2周时,实验组静脉桥内膜厚度较之对照组稍有减小(76.57±6.76μm vs 87.32±6.89μm),p=0.123,差异无统计学意义;4周时实验组较对照组内膜厚度明显变薄(45.39±9.41μm vs 134.07±18.80μm),P<0.05.p-AKT在实验组中的表达明显降低,P<0.05.相比于对照组,实验组大隐静脉平滑肌细胞增殖与迁移能力均受到抑制,P<0.05.结论: 3-HBA能抑制实验用新西兰兔自体移植静脉桥内膜增生,可能与其抑制AKT的磷酸化从而抑制平滑肌细胞增殖迁移有关.

Effects of 3-hydroxybenzaldehyde on intimal hyperplasia in autogenous vein grafts of rabbits

Objective: To study the effect of 3-HBA on autogenous vein grafts of rabbits.Methods: After jugular vein-to-artery bypass grafting surgery,24 healthy New Zealand white rabbits were randomly devided into the treatment group by injection of 3-HBA 100mg/(kg﹒d) via ear vein,and the control group by saline.After 2 or 4 weeks vein grafts were harvested.The thickness of neointima were mesuared by computer image analysis system,and western blot was used to detect the protein level p-AKT.Human saphenous vein smooth muscle cells were treated with 3-HBA or PBS after stimulated with PDGF for 24h.Cell proliferation was assayed by MTT and cell migration was observed by transwell chamber.Results: 2 weeks later,the neointima thickness of the treatment group(45.39±9.41μm)was inhibited compared with the control group (87.32±6.89μm) (P=0.123),and the difference was much higher at 4 weeks(76.57±6.76μm vs 134.07±18.80μm)(P<0.05).The expression of p-AKT was significantly lower(P<0.05).Cell proliferation and migration was statistically supressed(P<0.05).Conclusion: 3-HBA can inhibit hyperplasia of intimal thickness after venous transplantation of rabbits, which may be related to the lower expression of p-AKT and inhibition of smooth muscle cells proliferation.