儿童血清中性粒细胞明胶酶相关脂质运载蛋白与维生素A代谢指标相关性研究

【目的】 初步分析中性粒细胞明胶酶相关脂质运载蛋白(neutrophil gelatinase-associated lipocalin,NGAL)水平与维生素A代谢指标血清视黄醇、视黄醇结合蛋白 (retinol-binding protein,RBP)、甲状腺素运载蛋白(transthyretin,TTR)的关系。 【方法】 随机抽取研究现场8所幼儿园473名2~7岁(不足7岁)学龄前儿童纳入。分别利用HPLC法测血清视黄醇;采用ELISA法测NGAL与 RBP,采用免疫比浊法测TTR,并计算R-T指数RBP(mol/L)/TTR(mol/L)]。 【结果】 血清NGAL与血清视黄醇和R-T指数存在明显负相关关系(偏相关系数r分别为0.21和0.25;P均<0.000 1)。维生素A正常组儿童NGAL水平显著低于维生素A不正常组儿童(P<0.05)。以指标中位数为界,低R-T指数组儿童血清NGAL水平明显高于高R-T组儿童(P<0.05),但是高与低RBP组儿童之间以及高与低TTR组儿童之间血清NGAL水平差异均无统计学意义(P>0.05)。分别以血清NGAL水平的P₂₅,P₅₀以及P₇₅浓度为界,将研究对象分为Q1,Q2,Q3和Q4组,Q1组视黄醇水平和R-T指数均显著高于Q4组(P<0.05),血清RBP和TTR水平则无明显组间差异(P>0.05)。 【结论】 学龄前儿童血清NGAL水平与机体维生素A存在密切相关,维生素A可能可以通过抑制NGAL的表达而对抗NGAL对造血的抑制。

Relationship between serum neutrophil gelatinase-associated lipocalin and vitamin A metabolic index in preschool children

【Objective】 To explore the correlation of neutrophil gelatinase-associated lipocalin(NGAL) and vitamin A metabolic index,including serum retinol,serum retinol binding protein(RBP),serum transthyretin(TTR) and the molar ratio of RBP to TTRR-T index,RBP(mol/L)/TTR(mol/L)].【Methods】 About 473 preschool children with 2~7 years old were randomly selected from eight kindergartens,including 223 boys and 250 girls with(47.8±14.5) months.Serum retinol was measured by HPLC method,NGAL and RBP by ELISA and TTR by immunoturbidimetric method.【Results】 There were significant negative correlations between NGAL and retinol and R-T index(The partial correlation coefficients were-0.21 and-0.25;P all<0.000 1).The value of serum NGAL in children with normal vitamin A was markedly lower than that of children with unnormal vitamin A(P<0.05).Children with low R-T index level(cut by R-T index media value) had higher serum NGAL level than those with high R-T index level(P<0.05);However,no significant difference was observed between children with high and low RBP/TTR levels(P>0.05).The serum retinol and R-T index levels in children with Q1 RBP value group(cut by RBP 4 quartile level) was significantly higher than those of children with Q4 RBP value group(P<0.05),but not for serum RBP and TTR values(P>0.05).【Conclusion】 There is close relation between serum NGAL and vitamin A status in preschool children and vitamin A may be able to inhibit the expression of NGAL which against the inhibition of NGAL on hematopoietic system.