Heat shock 70 kDa protein cognate 5 involved in WSSV toleration of Litopenaeus vannamei |
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| Affiliation: | 1. Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering/South China Sea Bio-Resource Exploitation and Protection Collaborative Innovation Center (SCS-REPIC)/School of Marine Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China;2. School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China;3. State Key Laboratory for Biocontrol/MOE Key Laboratory of Aquatic Product Safety/Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China;1. Faculty of Aquatic and Fisheries Sciences, Kafr El Sheikh University, Egypt;2. Animal Husbandry and Animal Wealth Department, Faculty of Veterinary Medicine, Alexandria University, Egypt;3. Department of Physiology, Faculty of Veterinary Medicine, Kafr El Sheikh University, Egypt;1. Key Laboratory of Sustainable Utilization of Technology Research, Scientific Observing and Experimental Station of Fishery Resources for Key Fishing Grounds, MOA, Marine Fisheries Research Institute of Zhejiang, Zhejiang, Zhoushan, 316021, China;2. Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Hunan University of Arts and Science, Hunan, Changde, 415000, China;3. National Engineering Research Center of Marine Facilities Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, Zhejiang, Zhoushan, 316004, China;1. Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering/School of Marine Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China;2. School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China;3. State Key Laboratory for Biocontrol/MOE Key Laboratory of Aquatic Product Safety/Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China;1. Key Laboratory of Marine Resources and Coastal Engineering in Guangdong Province/School of Marine Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China;2. School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China;3. State Key Laboratory for Biocontrol/MOE Key Laboratory of Aquatic Product Safety/Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, PR China |
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| Abstract: | The expression levels of 97 unigenes encoding heat shock proteins of Litopenaeus vannamei was scanned, and ten of them were significantly induced by white spot syndrome virus (WSSV). Among these genes, heat shock 70 kDa protein cognate 5 (LvHSC70-5) was upregulated to the highest extent and subjected to further studies. Subcellular localization assay revealed that LvHSC70-5 was located in the mitochondria. Aside from WSSV infection, unfolded protein response activation and thermal stress could also upregulate LvHSC70-5. Results of reporter gene assay demonstrated that promoter of LvHSC70-5 was activated by L. vannamei heat shock factor protein 1, activating transcription factor 4 and thermal stress. A decrease in the expression of LvHSC70-5 could reduce the aggregation of proteins in hemocytes and the cumulative mortality of WSSV-infected L. vannamei. LvHSC70-5 in L. vannamei hemocytes was upregulated by mild thermal stress. In addition, mild thermal stress, decreased the copy number of WSSV in shrimp muscle and the cumulative mortality of WSSV-infected L. vannamei. Therefore, collecting results suggested that LvHSC70-5 should be involved in WSSV toleration of shrimp L. vannamei. |
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| Keywords: | ER-stress Unfolded protein response Heat shock protein cognate 5 White spot syndrome virus |
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