New immunomodulatory role of neuropeptide Y (NPY) in Salmo salar leucocytes期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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New immunomodulatory role of neuropeptide Y (NPY) in Salmo salar leucocytes

Institution:	1. Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, School of Dentistry, Seoul National University, Seoul 110-749, Republic of Korea;2. Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea;3. Division of High-risk Pathogen Research, Center for Infectious Diseases, Korean National Institute of Health, Cheongwon-gun, Chungbuk 363-951, Republic of Korea

Abstract:	Neuropeptide Y (NPY) plays different roles in mammals such as: regulate food intake, memory retention, cardiovascular functions, and anxiety. It has also been shown in the modulation of chemotaxis, T lymphocyte differentiation, and leukocyte migration. In fish, NPY expression and functions have been studied but its immunomodulatory role remains undescribed. This study confirmed the expression and synthesis of NPY in S. salar under inflammation, and validated a commercial antibody for NPY detection in teleost. Additionally, immunomodulatory effects of NPY were assayed in vitro and in vivo. Phagocytosis and superoxide anion production in leukocytes and SHK cells were induced under stimulation with a synthetic peptide. IL-8 mRNA was selectively and strongly induced in the spleen, head kidney, and isolated cells, after in vivo challenge with NPY. All together suggest that NPY is expressed in immune tissues and modulates the immune response in teleost fish.

Keywords:	Neuropeptide Y Immunomodulation Phagocytosis Cytokines BSA"} {"#name":"keyword" "$":{"id":"kwrd0040"} "$$":[{"#name":"text" "_":"bovine serum albumin bp"} {"#name":"keyword" "$":{"id":"kwrd0050"} "$$":[{"#name":"text" "_":"base pairs cAMP"} {"#name":"keyword" "$":{"id":"kwrd0060"} "$$":[{"#name":"text" "_":"cyclic adenosine monophosphate DAPI"} {"#name":"keyword" "$":{"id":"kwrd0070"} "$$":[{"#name":"text" "_":"4′6-diamidino-2-phenylindole DC"} {"#name":"keyword" "$":{"id":"kwrd0080"} "$$":[{"#name":"text" "_":"dendritic cell DMSO"} {"#name":"keyword" "$":{"id":"kwrd0090"} "$$":[{"#name":"text" "_":"dimethyl sulfoxide DPI"} {"#name":"keyword" "$":{"id":"kwrd0100"} "$$":[{"#name":"text" "_":"dibenziodolium chloride ELISA"} {"#name":"keyword" "$":{"id":"kwrd0110"} "$$":[{"#name":"text" "_":"enzyme-linked immunosorbent assay FCS"} {"#name":"keyword" "$":{"id":"kwrd0120"} "$$":[{"#name":"text" "_":"fetal calf serum fMLP"} {"#name":"keyword" "$":{"id":"kwrd0130"} "$$":[{"#name":"text" "_":"N-formyl-methionyl-leucyl-phenylalanine HK"} {"#name":"keyword" "$":{"id":"kwrd0140"} "$$":[{"#name":"text" "_":"head kidney iDC"} {"#name":"keyword" "$":{"id":"kwrd0150"} "$$":[{"#name":"text" "_":"immature dendritic cell IL"} {"#name":"keyword" "$":{"id":"kwrd0160"} "$$":[{"#name":"text" "_":"interleukin L15"} {"#name":"keyword" "$":{"id":"kwrd0170"} "$$":[{"#name":"text" "_":"Leibovitz medium LPS"} {"#name":"keyword" "$":{"id":"kwrd0180"} "$$":[{"#name":"text" "_":"lipopolysaccharide MS-222"} {"#name":"keyword" "$":{"id":"kwrd0190"} "$$":[{"#name":"text" "_":"tricaine methane-sulfonate NBT"} {"#name":"keyword" "$":{"id":"kwrd0200"} "$$":[{"#name":"text" "_":"nitroblue tetrazolium NK"} {"#name":"keyword" "$":{"id":"kwrd0210"} "$$":[{"#name":"text" "_":"natural killer NPY"} {"#name":"keyword" "$":{"id":"kwrd0220"} "$$":[{"#name":"text" "_":"neuropeptide Y PAMPs"} {"#name":"keyword" "$":{"id":"kwrd0230"} "$$":[{"#name":"text" "_":"pathogen-associated molecular patterns PBS"} {"#name":"keyword" "$":{"id":"kwrd0240"} "$$":[{"#name":"text" "_":"phosphate buffer saline PMA"} {"#name":"keyword" "$":{"id":"kwrd0250"} "$$":[{"#name":"text" "_":"phorbol myristate acetate Poly I:C"} {"#name":"keyword" "$":{"id":"kwrd0260"} "$$":[{"#name":"text" "_":"polyinosinic-polycytidylic acid PP"} {"#name":"keyword" "$":{"id":"kwrd0270"} "$$":[{"#name":"text" "_":"pancreatic peptide prepro-NPY"} {"#name":"keyword" "$":{"id":"kwrd0280"} "$$":[{"#name":"text" "_":"precursor polypeptide PY"} {"#name":"keyword" "$":{"id":"kwrd0290"} "$$":[{"#name":"text" "_":"peptide Y PYY"} {"#name":"keyword" "$":{"id":"kwrd0300"} "$$":[{"#name":"text" "_":"peptide YY ROS"} {"#name":"keyword" "$":{"id":"kwrd0310"} "$$":[{"#name":"text" "_":"reactive oxygen species SHK-1"} {"#name":"keyword" "$":{"id":"kwrd0320"} "$$":[{"#name":"text" "_":"cell line derived from Atlantic salmon head kidney SSP9"} {"#name":"keyword" "$":{"id":"kwrd0330"} "$$":[{"#name":"text" "$$":[{"#name":"__text__" "_":"cell line derived from pronephros of the "} {"#name":"italic" "_":"Salmo salar
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