Autophagy induced by infectious hematopoietic necrosis virus inhibits intracellular viral replication and extracellular viral yields in epithelioma papulosum cyprini cell line |
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| Affiliation: | 1. Heilongjiang River Fishery Research Institute Chinese Academy of Fishery Sciences, Harbin 150070, PR China;2. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001, China;1. Virginia Institute of Marine Science, College of William and Mary, PO Box 1346, Gloucester Point, VA 23062, United States;2. USGS Western Fisheries Research Center, 6505 NE 65th St., Seattle, WA 98115, United States;3. University of Washington, Department of Biology, Box 351800, Seattle, WA 98195, United States;1. College of Animal Science and Veterinary Medicine, Jilin University, Jilin 130062, People’s Republic of China;2. Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen 518045, People’s Republic of China;3. Institute of Military Veterinary Medicine, Academy of Military Medical Sciences of PLA, Jilin 130117, People’s Republic of China;4. Shenzhen Supervision and Testing Center for Quality and Safety of Agri-products, Shenzhen 518005, People’s Republic of China;5. Jilin Academy of Fishery Science, Jilin 130033, People’s Republic of China;1. Heilongjiang River Fishery Research Institute Chinese Academy of Fishery Sciences, Harbin 150070, China;2. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001, China;1. Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, China;2. College of Animal Science and Technology, Northeast Agricultural University, Harbin, Heilongjiang, China;1. Laboratory of Fish Diseases, Department of Aquaculture, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Songfa Street No. 43, Daoli District, Harbin, 150070, China;2. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China;1. Heilongjiang River Fishery Research Institute Chinese Academy of Fishery Sciences, Harbin 150070, PR China;2. Western Fisheries Research Center, USGS, Seattle, WA 98115, USA;3. Clear Springs Foods Inc., Research Division, Buhl, ID, USA;4. Benxi Agrimarine Company Limited, Benxi 117000, PR China |
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| Abstract: | Infectious hematopoietic necrosis virus (IHNV) is a common pathogen that causes severe disease in the salmonid aquaculture industry. Recent work demonstrated that autophagy plays an important role in pathogen invasion by activating innate and adaptive immunity. This study investigated the relationship between IHNV and autophagy in epithelioma papulosum cyprini cells. The electron microscopy results show that IHNV infection can induce typical autophagosomes which are representative structures of autophagy activation. The punctate accumulation of green fluorescence-tagged microtubule-associate protein 1 light chain 3 (LC3) and the protein conversion from LC3-I to LC3-II were respectively confirmed by confocal fluorescence microscopy and western blotting. Furthermore, the effects of autophagy on IHNV replication were also clarified by altering the autophagy pathway. The results showed that rapamycin induced autophagy can inhibit both intracellular viral replication and extracellular viral yields, while autophagy inhibitor produced the opposite results. These findings demonstrated that autophagy plays an antiviral role during IHNV infection. |
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| Keywords: | Infectious hematopoietic necrosis virus Autophagy LC3 Antiviral response |
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