Selenium accelerates chicken dendritic cells differentiation and affects selenoproteins expression |
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| Affiliation: | 1. INSERM, UMRS 1138, Centre de Recherche des Cordeliers, 15 rue de l''École de Médecine, 75006, Paris, France;2. Assistance Publique Hôpitaux de Paris, Bichat Hospital, DHU FIRE, Department of Diabetology, Endocrinology and Nutrition, 46 rue Henri Huchard, 75018, Paris, France;3. Laboratório de Endocrinologia Celular e Molecular (LIM-25), Faculdade de Medicina da Universidade de São Paulo (FMUSP), Avenida Dr. Arnaldo 455, CEP 01246903, São Paulo, SP, Brazil;4. INSERM, Research Unit 773, 16 rue Henri Huchard, 75018, Paris, France;5. Assistance Publique Hôpitaux de Paris, Bichat Hospital, Department of Biochemistry, 46 rue Henri Huchard, 75018, Paris, France;6. INSERM, Research Unit 1137 — IAME, 16 rue Henri Huchard, 75018, Paris, France;7. Université Paris Diderot, Sorbonne Paris Cité, UFR de Médecine, 16 rue Henri Huchard, 75018, Paris, France;8. Centre Hospitalier Universitaire de Poitiers, Department of Endocrinology and Diabetology, 2 rue de la Milétrie, 86021, Poitiers, France;9. INSERM, Research Unit 1082, 2 rue de la Milétrie, 86021, Poitiers, France;10. INSERM, CIC 1402, 2 rue de la Milétrie, 86021, Poitiers, France;11. Université de Poitiers, UFR de Médecine et Pharmacie, 6 rue de la Milétrie, 86073, Poitiers, France;12. Centro de Terapia Celular e Molecular (NUCEL/NETCEM) da FMUSP, Avenida Dr. Arnaldo 455, CEP 01246903, São Paulo, SP, Brazil |
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| Abstract: | Selenium (Se) promotes immune cell differentiation and improves immune response. Antigen-presenting cells such as dendritic cells (DCs) play an important role in immune system, however, the impact of Se on DCs is still unclear. In this study, we successfully induced and cultured chicken DCs from peripheral blood mononuclear cells by incubating mononuclear cells with 50 ng/mL recombinant chicken granulocyte-macrophage colony stimulating factor and 10 ng/mL recombinant chicken interleukin-4 for total 9 days. In + Se group, we added 10−7 mol/L sodium selenite from the first day of cell culture. The results showed that Se supplementation expedited and increased the expression of cell surface markers including CD11c, CD40, CD86, and MHC II. Principal component analysis showed that the expression of selenoproteins SelW, SelK, Dio3, GPX1, GPX2, SelN, SelS, SelH in chicken DCs was highly correlated, and SelW had highest correlation with the cell surface markers MHC II and CD11c. In conclusion, Se accelerates the differentiation and maturation of chicken DCs. Se regulates the differentiation and maturation of chicken DCs by selenoproteins. Selenoproteins has closely correlated to surface markers of chicken DCs. |
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| Keywords: | Selenium Selenoproteins Chicken Dendritic cells |
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