Tissue differences in DNA methylation changes at AHRR in full term low birth weight in maternal blood,placenta and cord blood in Chinese |
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| Affiliation: | 1. Department of Medical Statistics and Epidemiology, Guangzhou Key Laboratory of Environmental Pollution and Health Assessment, Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-sen University, Guangzhou, Guangdong, China;2. Department of Population Medicine, Harvard Medical School, Harvard Pilgrim Health Care Institute, 401 Park Drive, Suite 401, Boston, MA, USA;3. Diabetes Center, Massachusetts General Hospital, 50 Staniford Street, Boston, MA, USA;4. Department of Medicine, Université de Sherbrooke, 3001 12th Avenue North, Sherbrooke, Québec, Canada;5. Centre de recherche du Centre Hospitalier Universitaire de Sherbrooke, 3001 12th Avenue North, Wing 9, Door 6, Sherbrooke, Québec, Canada;6. Division of Behavioral Medicine, Department of Pediatrics, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, NY, USA;7. Department of Cancer Prevention Research, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China;1. Laboratory of Medical Genetics, Medical Research Institute, Cheil General Hospital and Women''s Healthcare Center, Seoul, South Korea;2. Department of Obstetrics and Gynecology, Cheil General Hospital and Women''s Healthcare Center, Catholic Kwandong University College of Medicine, Seoul, South Korea;1. Arkansas Children''s Nutrition Center, Little Rock, AR, USA;2. Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR, USA;3. Arkansas Children''s Hospital, Little Rock, AR, USA;4. Arkansas Children''s Research Institute, Little Rock, AR, USA;1. Division of Nutrition, St. John''s Research Institute, St. John''s National Academy of Health Sciences, Bangalore, India;2. Department of Pathology, St John''s Medical College, St. John''s National Academy of Health Sciences, Bangalore, India;3. Department of Obstetrics and Gynecology, St John''s Medical College, St. John''s National Academy of Health Sciences, Bangalore, India;4. Division of Epidemiology and Biostatistics, St. John''s Research Institute, St. John''s National Academy of Health Sciences, Bangalore, India;5. Division of Molecular Medicine, St. John''s Research Institute, St. John''s National Academy of Health Sciences, Bangalore, India;1. Servicio de Neonatología, BCNatal-Hospital Sant Joan de Déu, Barcelona, Spain;2. Imprinting and Cancer Group, Cancer Epigenetic and Biology Program (PEBC), Institut d''Investigació Biomedica de Bellvitge, Hospital Duran i Reynals (IDIBELL), Barcelona, Spain;1. Department of Neonatology, Dr Sami Ulus Maternity and Children Research and Training Hospital, University of Health Sciences, Ankara, Turkey;2. Department of Neonatology, Memorial Dicle Hospital, Diyarbakır, Turkey;3. Intergen Genetics Center, Ankara, Turkey;4. Department of Obstetrics and Gynecology, Dr Sami Ulus Maternity and Children Research and Training Hospital, University of Health Sciences, Ankara, Turkey |
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| Abstract: | IntroductionVery few study addressed the relationship between Aryl-hydrocarbon receptor repressor (AHRR) DNA methylation and low birth weight, especially in multiple tissues of mother-infant pairs. In this study, we aimed to investigate AHRR DNA methylation modification in cord blood, placenta and maternal blood between full term low birth weight (FT-LBW) and full term normal birth weight (FT-NBW) newborns.MethodsWe enrolled 90 FT-LBW and 90 FT-NBW mother-infant pairs, of which all placenta and cord blood samples were collected while 45 maternal blood samples of each group were collected. We measured AHRR DNA methylation (Chr5: 373013–373606) using Sequenom MassARRAY, and assessed associations between AHRR DNA methylation and FT-LBW using logistic regression adjusting for maternal age, education, family income, delivery mode, and passive smoking.ResultsFT-LBW babies had 3% lower methylation at Chr5: 373378 (CpG 13) in cord blood, and 4–9% higher methylation levels at Chr5: 373315, 373378, 373423, 373476 and 373490/373494 (CpG 10; 13; 15; 16; 17/18 respectively) in maternal blood, comparing with FT-NBW. The methylation of Chr5: 373378 (CpG 13) remained significant association with FT-LBW both in cord blood (OR = 0.90; 95% CI: 0.82, 0.98) and maternal blood (OR = 1.14; 95% CI: 1.04, 1.25) further adjusting for each other in the same model. We observed no significant difference at any CpG sites in placenta.DiscussionAHRR DNA methylation of cord and maternal blood might be independently associated with FT-LBW in different ways. |
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| Keywords: | DNA methylation Low birth weight Cord blood Placenta Maternal blood |
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