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Distinct T cell subsets in adipose tissue are associated with obesity |
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| Authors: | Martha E Haugstøyl Martin Cornillet Kristina Strand Natalie Stiglund Dan Sun Laurence Lawrence-Archer Iren D Hjellestad Ernesto Sparrelid Christian Busch Jøran Hjelmesæth Jens K Hertel Andrea Ponzetta Gunnar Mellgren Johan Fernø Niklas K Björkström |
| Institution: | 1. Hormone Laboratory, Department of Medical Biochemistry and Pharmacology, Haukeland University Hospital, Bergen, Norway
Mohn Nutrition Research Laboratory, Department of Clinical Science, University of Bergen, Bergen, Norway;2. Center for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden;3. Hormone Laboratory, Department of Medical Biochemistry and Pharmacology, Haukeland University Hospital, Bergen, Norway Department of Medicine, Haukeland University Hospital, Bergen, Norway;4. Division of Surgery, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden;5. Plastikkirurg1, Bergen, Norway;6. Morbid Obesity Centre, Department of Medicine, Vestfold Hospital Trust, Tønsberg, Norway Department of Endocrinology, Morbid Obesity and Preventive Medicine, Institute of Clinical Medicine, University of Oslo, Oslo, Norway;7. Morbid Obesity Centre, Department of Medicine, Vestfold Hospital Trust, Tønsberg, Norway;8. Hormone Laboratory, Department of Medical Biochemistry and Pharmacology, Haukeland University Hospital, Bergen, Norway Mohn Nutrition Research Laboratory, Department of Clinical Science, University of Bergen, Bergen, Norway These authors contributed equally to this work |
| Abstract: | Adipose tissue inflammation is a driving factor for the development of obesity-associated metabolic disturbances, and a role of adipose tissue T cells in initiating the pro-inflammatory signaling is emerging. However, data on human adipose tissue T cells in obesity are limited, reflected by the lack of phenotypic markers to define tissue-resident T cell subsets. In this study, we performed a deep characterization of T cells in blood and adipose tissue depots using multicolor flow cytometry and RNA sequencing. We identified distinct subsets of T cells associated with obesity expressing the activation markers, CD26 and CCR5, and obesity-specific genes that are potentially engaged in activating pro-inflammatory pathway, including ceramide signaling, autophagy, and IL-6 signaling. These findings increase our knowledge on the heterogeneity of T cells in adipose tissue and on subsets that may play a role in obesity-related pathogenesis. |
| Keywords: | adipose tissue multicolor flow cytometry obesity obesity-specific genes T cells |