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MiR-136 contributes to pre-eclampsia through its effects on apoptosis and angiogenesis of mesenchymal stem cells
Institution:1. Obstetrical Department, No.215 Hospital of Shanxi Nuclear Industry, Xianyang, Shaanxi, 712000, China;2. Obstetrical Department, Yan''an University Affiliated Hospital, Yan''an, Shaanxi, 716000, China;3. The 2nd Department of Obstetrical, Northwest Women and Children''s Hospital, Xian, Shaanxi, 710000, China;4. Obstetrical Department, Northwest Women and Children''s Hospital, Xian, Shaanxi, 710000, China;5. Gynaecology and Obstetrics, 4th (Xing Yuan) Hospital of Yulin, Yulin, Shaanxi, 719000, China;6. Gynaecology and Obstetrics, Xian NO.1 Hospital, Xian, Shaanxi, 710000, China;7. Obstetrical Department, Shaanxi Baoji Maternal and Child Health Hospital, Baoji 721000, China;8. Obstetrical Department, Yan''an People''s Hospital, Yanan, Shaanxi, 716000, China;1. Department of Epidemiology, School of Public Health, University of Washington, Seattle, WA, United States;2. Center for Perinatal Studies, Swedish Medical Center, Seattle, WA, United States;3. Department of Epidemiology, Harvard T.H. Chan School of Public Health, Boston, MA, United States;1. Institute of Arthritis Research, 2220 East 25th St, Idaho Falls, ID 83404, USA;2. Idaho State University, Department of Biology, 921 South 8th Avenue, Pocatello, ID 83201, USA;1. Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia;2. Institute of Pathophysiology, Faculty of Medicine, Comenius University, Bratislava, Slovakia;3. Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia;1. Department of Obstetrics and Gynecology, The First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, Chongqing 400016, China;2. Chongqing Health Center For Women and Children, No. 64 Jintang Street, Yuzhong District, Chongqing 400013, China;3. Laboratory of Lipid and Glucose Research, The First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, Chongqing 400016, China;1. Institute for the Application of Nuclear Energy, INEP, University of Belgrade, Banatska 31b, 11080 Belgrade, Serbia;2. Faculty of Medicine, University of Belgrade, Doktora Suboti?a 8, 11000 Belgrade, Serbia;1. Department of Molecular Medicine and Anatomy, Nippon Medical School, Tokyo 113-8602, Japan;2. Department of Obstetrics and Gynecology, Jichi Medical University, Tochigi 329-0498, Japan;3. Department of Obstetrics and Gynecology, Faculty of Medicine, University of Toyama, Toyama 930-0194, Japan
Abstract:IntroductionMesenchymal stem cells (MSCs) play an important role in the pathology of preeclampsia (PE). The survival of MSCs and angiogenesis in the maternal-fetal interface are important for a successful pregnancy. MicroRNA-136 (miR-136) is highly expressed in decidua-derived MSCs (MSCs) from PE compared with healthy donors (NC). The role of the MSCs aberrant expressed miR-136 in PE development is still unclear. In the present study, we examined the impact of miR-136 on the survival of MSCs and angiogenesis in the maternal-fetal interface.MethodsMSCs were extracted and transfected with miR-136 mimic and interfering RNAs using lipofectamine-2000. Then cell apoptosis were tested using flow cytometry. HUVEC tube formation ability was tested on Matrigel co-cultured with conditioned MSCs supernatants.ResultsHigh level of miR-136 could suppress cell proliferation and promote apoptosis of MSCs through targeting BCL2. It could also impairs HUVEC capillary formation by suppressing VEGF.DiscussionMiR-136 significantly increase the apoptosis and suppress the proliferation of MSCs. It could also inhibit the capillary formation and trophoblast cell invasion. These data suggest that decidua-derived miR-136 that is increased in PE is a potential causal factor of PE.
Keywords:MiR-136  Mesenchymal stem cells  Preeclampsia  Apoptosis  Angiogenesis  BCL2  PE"}  {"#name":"keyword"  "$":{"id":"kwrd0045"}  "$$":[{"#name":"text"  "_":"preeclampsia  MSCs"}  {"#name":"keyword"  "$":{"id":"kwrd0055"}  "$$":[{"#name":"text"  "_":"mesenchymal stem cells  NC"}  {"#name":"keyword"  "$":{"id":"kwrd0065"}  "$$":[{"#name":"text"  "_":"healthy donors  VEGF"}  {"#name":"keyword"  "$":{"id":"kwrd0075"}  "$$":[{"#name":"text"  "_":"vascular endothelial growth factor  HUVEC"}  {"#name":"keyword"  "$":{"id":"kwrd0085"}  "$$":[{"#name":"text"  "_":"human umbilical vein endothelial cells
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