5-氮-2-脱氧胞苷联合曲古抑菌素A在体内、体外对人胆管癌细胞QBC-939增殖的影响

目的 研究5-氮-2-脱氧胞苷（5-Aza-Cdr）和曲古抑菌素A（TSA）在体内、体外对人胆管癌细胞QBC-939生长的影响,探讨其应用于胆管癌生物学治疗的价值.方法 应用生长曲线、MTT、流式细胞仪、胆管癌裸鼠种植模型,检测不同浓度的5-Aza-Cdr和TSA,以及联合化疗药物对QBC-939体内、体外增殖的影响.结果 5-Aza-Cdr和TSA对QBC-939有明显的抑制作用,呈浓度、时间依赖性.流式细胞仪检测细胞周期主要阻滞于G1/S期,凋亡不明显.QBC-939经处理后裸鼠体内种植瘤生成率降低,荷瘤小鼠给予5-Aza-Cdr,TSA和氟尿嘧啶后肿瘤生长速度减慢、部分体积减小.结论 5-Aza-Cdr和TSA在体内、体外能抑制人胆管癌细胞QBC-939的生长,可能为胆管癌的生物学治疗提供一种新的思路.

Effects of 5-Aza-2deoxycydine in combination with trichostatin A on QBC-939 in vivo and in vitro

Objective To investigate the effects of 5-Aza-2deoxycydine(5-Aza-Cdr)in combi- nation with trichostatin A(TSA)on QBC-939 in vivo and in vitro to explore its possibility in biologi- cal treatment of cholangiocarcinoma.Methods The effects of 5-Aza-Cdr in combination with TSA on QBC-939 cell line in vivo were determined by MTT assay,growth curve and flow cytometry.Their effects on the cell line in vitro were investigated through establishing the nude mice model.Results 5- Aza-Cdr and TSA showed inhibitory effects on the proliferation of QBC-939 in dose-and time-depend- ent manner.Cell cycle was blocked in the G1/S phase in vivo,and tumor occurring rate was lower in the 5-Aza-Cdr,TSA and 5-Flu pre-treated cells in vitro.Conclusions 5-Aza-Cdr and TSA can sup- press cell proliferation and have the co-operative effect with some chemotherapeutic agents on QBC-939 in vivo and in vitro,which can be a new approach for treatment of cholangioearcinoma.