血管内皮细胞生长因子和血管生成素-2在喉癌中表达及意义

目的：检测血管内皮细胞生长因子和血管生成素-2在喉癌中的表达，探讨它们与喉癌生物学行为及临床病理学特征间的关系。方法：采用免疫组织化学S-P法，检测喉癌84例、不典型增生5例及声带息肉5例组织中VEGF和Ang-2表达。统计分析其表达与喉癌临床病理因素的关系及相关性。结果：喉癌84例中VEGF总阳性率70.2%。增加不同组间表达率，不同分化程度喉癌间的表达差异有统计学意义（χ2=17.323, P＜0.05）；不同分期喉癌间表达差异有统计学意义（χ2=11.489, P＜0.05）；有无淋巴结转移两组间差异有统计学意义（χ2=18.748, P＜0.05）。Ang-2总阳性47例（56.0%）。高、中、低分化喉癌中阳性率分别为38.8%、68.8%和100%；不同分化程度喉癌间的表达差异有统计学意义（χ2=22.330, P＜0.05）；淋巴结转移组和无淋巴结转移组阳性率分别为78.6%和48.6%组比较差异有统计学意义（χ2=14.880, P＜0.05）。结论：喉癌中VEGF与Ang-2呈正相关，两者联合检测有助于揭示喉癌发生、发展、转移的机制。

Expressions of VEGF and Ang-2 in laryngeal carcinoma and their significance

Objective：To investigate their possible relationships to biological behavior and clinicopathological char-acteristics in laryngeal carcinoma by detecting the expressions of vascular endothelial growth factor （VEGF） and Ang-2 in laryngeal carcinoma. Methods：S-P immunohistochemical staining was used to examine the expressions of VEGF and Ang-2 in 84 laryngeal carcinoma and 5 vocal cord polyps （VCP） and 5 atypical hyperplasia tissues. The relationships among the expressions of VEGF and Ang-2 in laryngeal carcinoma and clinical pathologic characteristics were analyzed by statistical methods. Results：The total expression of VEGF was 70.2%in laryngeal carcinoma. The expression had a difference in dif-ferentiated degrees of laryngeal carcinoma （χ2=17.323, P〈0.05）.And there was a significant difference in the expression of different stages （χ2=11.489, P〈0.05）.The rate of the expression of VEGF in tissues with lymphatic metastasis and in that without lymphatic metastasis was different, and their difference was significant （χ2=18.748, P〈0.05）.The total expression of Ang-2 was 51.2%（47/84） in laryngeal carcinoma. The percentage of Ang-2 was 38.8%（19/49）, 68.8%（22/32） and 100%（3/3） in well-differentiated, moderately-differentiated and poorly-differentiated. The expression had a difference between dif-ferentiated degrees of laryngeal carcinoma （χ2=23.330, P〈0.05）. The rate of the expression of Ang-2 in tissues with lym-phatic metastasis and in that without lymphatic metastasis was 78.6%（11/14）and 48.6%（34/70）,and their difference was significant （χ2=14.880, P〈0.05）. Conclusions： The expression of Ang-2 was positively correlated with VEGF, which sug-gests that they may facilitate the development of laryngeal carcinoma. Combined analysis of these two proteins might be useful both in the disclosure of the mechanism of the occurrence and development of laryngeal carcinoma.