斑秃患者外周血单一核细胞中Th1/Th2型细胞因子的表达

【摘　要】:目的:探讨斑秃患者外周血单一核细胞(PBMC)中Th1/Th2型细胞因子的表达状况与斑秃发病的关系。方法:用逆转录—聚合酶链反应(RT-PCR)分别检测18例轻型斑秃、24例重型斑秃患者及20例正常人外周血单一核细胞(PBMC)经PHA刺激后具有代表性的Th1型细胞因子：γ干扰素（IFN-γ）和肿瘤坏死因子β（TNF-β）和具有代表性的Th2细胞因子白介素4（ IL-4）、白介素10（IL-10）的表达水平。结果:经PHA刺激后轻型斑秃患者PBMC中Th1型细胞因子(IFN-γ、TNF-β)基因表达水平明显升高,与正常对照组比较有显著性差异(P<0. 05);Th2型细胞因子(IL-4、 IL-10)基因表达水平下降,显著低于正常对照组(P<0. 05)；重型斑秃患者PBMC中Th1型细胞因子(IFN-γ、IL-12)基因表达不仅显著高于正常对照组(P<0. 05),而且还高于轻型斑秃病人(P<0. 05);Th2型细胞因子(IL-4、IL-10)基因表达显著低于正常对照组(P<0. 05),与轻型斑秃病人比较有显著性差异。结论:斑秃患者PBMC中Th1/Th2型细胞因子基因表达失衡,表现以Th1型细胞因子基因过度表达为特征的Th1型反应,可能参与了斑秃的发病机制。 关键词:斑秃;细胞因子;基因表达; γ-干扰素； 肿瘤坏死因子-β； 白介素-4 ； 白介素-10 ；外周血单个核细胞;

Expressin of Th1/Th2 type cytokines in peripheral blood mononuclear cells from patients with alopecia areata

Abstract] 　Objective: To investigate the relationship between expression ofTh1/Th2 types cytokines( IFN-γ, TNF-β、IL-10、IL-4) mRNA in PBMC of the patients with alopecia areata; to investigate their roles in the pathogenesis of AA..Methods:Apply RT-PCR technique to detect the mRNA expression ofTh1 types cytokines（ IFN-γ、 TNF-β）、Th2 types cytokines （IL-10、IL-4）in PBMC of the patients with alopecia areata stimulated by PHA. Results:The mRNA expression of Th1 type cytokines in PHA-stimulated PBMC of the patients with localized alopecia areata patients were increased compared with healthy controls(P<0. 05), the mRNA expression of Th2 type cytokines(IL-4、 IL-10) were decreased compared with healthy controls (P<0. 05). the patients with extensive alopecia areata were increased compared with healthy controls (P<0. 01)and localized AA (P<0. 05), the mRNA expression ofTh2 type cytokines (IL-4、IL-10) were decreased compared with healthy controls and localized AA. (P<0. 05) Conclusion：the expression of Th1/Th2 types cytokines were unbalance in AA.there were over-expression of Th1 type cytokines in the PBMC of alopecia areata ,this may play an important role in pathogenesis of AA. Keywords: alopecia areata; cytokines ； gene expression ； IFN-γ、；TNF-β； IL-4；、 IL-10；PBMC.