| 新型乙型肝炎病毒核心区核酸疫苗的免疫原性研究 |
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| 引用本文： | 邢益平,黄祖瑚,王世霞,蔡洁,李军,卢山. 新型乙型肝炎病毒核心区核酸疫苗的免疫原性研究[J]. 中华肝脏病杂志, 2004, 12(6): 338-340 |
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| 作者姓名： | 邢益平 黄祖瑚 王世霞 蔡洁 李军 卢山 |
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| 作者单位： | 1. 210029,南京,南京医科大学第一附属医院感染病科
2. 美国马萨诸塞州大学医学院 |
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| 基金项目： | 江苏省135工程重点医学人才基金(135—044)，江苏省人事厅留学回国人员基金(272IA0203) |
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| 摘 要： | 目的观察新型乙型肝炎病毒(HBV)核心抗原(HBcAg)核酸疫苗的免疫原性。方法应用新型人体应用载体质粒pSW389l构建HBcAg核酸疫苗(pSW3891／HBc)，对照组和实验组Balb／c小鼠分别以基因枪法免疫对照载体质粒(PSW3891)和HBcAg核酸疫苗，采用酶联免疫吸附试验检测抗HBc，乳酸脱氢酶(LDH)释放测定法检测小鼠HBcAg特异性cTL杀伤活性。结果HBcAg核酸疫苗可在体外293T细胞中高效表达，免疫小鼠后可产生高滴度抗HBc(1：97200)，免疫鼠脾细胞HBc特异性CTL杀伤活性达73．25％。结论新型HBcAg核酸疫苗在Balb／c小鼠实验中表现出良好的体液和细胞免疫原性。
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| 关 键 词： | 乙型肝炎病毒 核酸疫苗 免疫原性 核心抗原 体液免疫 细胞免疫 |
| 修稿时间： | 2003-06-14 |
Immunogenicity of new DNA vaccine encoding for hepatitis B virus core antigen |
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| XING Yi-ping,HUANG Zu-hu,WANG Shi-xia,CAI Jie,LI Jun,LU Shan. Immunogenicity of new DNA vaccine encoding for hepatitis B virus core antigen[J]. Chinese journal of hepatology, 2004, 12(6): 338-340 |
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| Authors: | XING Yi-ping HUANG Zu-hu WANG Shi-xia CAI Jie LI Jun LU Shan |
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| Affiliation: | Department of Infectious Diseases, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China. |
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| Abstract: | Objective To observe immunogenicity of new DNA vaccine encoding for hepatitis B virus coreantigen (HBcAg). Methods A new DNA vaccine (pSW3891/HBc) encoding for hepatitis B virus core antigen wasconstructed using plasmid pSW3891 which can be used in human. Control and experiment groups of Balb/c mice wereimmunized with pSW3891 or pSW3891/HBc by gene gun. Anti-HBc in sera of mice was tested by ELISA (enzyme linkedimmune sorbent assay). Specific cytotoxicity of cytotoxic T lymphocyte (CTL) of mice was detected by LDH releaseassay. Results pSW3891/HBc can express in 293T cell line effectively. Mice immunized with pSW3891/HBc showedstrong anti-HBc response (ending point>1: 97 200) and specific high cytotoxicity of CTL (>73.25%). ConclusionpSW3891/HBc induced significantly humoral and cellular immune responses in Balb/c mice. |
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| Keywords: | DNA vaccine Hepatitis B Hepatitis B virus core antigen Immunogenicity |
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