吡格列酮对高糖高胰岛素诱导的心肌细胞肥大的影响

目的　利用体外培养的乳鼠心肌细胞 ,观察吡格列酮对高浓度葡萄糖与高浓度胰岛素共同诱导的肥大心肌细胞的影响 ,进一步推测吡格列酮对糖尿病并发心肌肥大的可能作用及作用机制。方法　以培养的乳鼠心肌细胞为模型分组给药后 ,用Lowrys法测心肌细胞的蛋白质含量 ;用 3 H]leucine标记法测定心肌细胞蛋白的合成 ;利用计算机图像分析系统测心肌细胞的体积 ;用显微镜目镜计数心肌细胞搏动的频率。结果　 10 μmol·L-1浓度的吡格列酮在对高糖高胰岛素诱导的肥大心肌细胞的蛋白含量、蛋白合成及体积均有显著的抑制作用。其抑制肥大的效果比维拉帕米更为明显 ;同时观察到吡格列酮同维拉帕米一样有抑制心肌细胞搏动的作用。结论　吡格列酮能有效抑制高糖高胰岛素诱导的心肌细胞肥大。这种作用可能是通过作用于PPARγ来实现的。

Effects of pioglitazone on hypertrophic myocardial cells induced with high glucose and high insulin

Aim To study the effects of pioglitazone on the hypertrophic myocardial cells induced with high glucose and high insulin. Methods Using cultured myocardial cells as a model, The protein content was assayed with Lowrys method; The contracting frequency was counted by the inverted microscope;The protein synthesis was assayed with leucine intake method;The cardiomyocytes volumes was measured by computer photograph analysis system; the cellular hypertrophy was observed. Results Compared with verapamil, Pioglitazone significantly inhibited the protein content, cellular protein synthesis and volumes of cultured hypertrophic myocardial cells induced with high glucose and high insulin. Meanwhile pioglitazone and verapamil inhibited myocardial cells contracting frequency too. Conclusions Pioglitazone inhibited cardiac hypertrophy of cultured myocardial cells induced with high glucose and high glucose. The mechanism of the effect of pioglitazone on cardiac hypertrophy needs further study.