| GAGE-7自身抗体在食管鳞状细胞癌中的表达 |
| |
| 引用本文： | 王皓,钟理,徐家馨,张小刚,王建飞. GAGE-7自身抗体在食管鳞状细胞癌中的表达[J]. 世界华人消化杂志, 2010, 18(10) |
| |
| 作者姓名： | 王皓 钟理 徐家馨 张小刚 王建飞 |
| |
| 作者单位： | 1. 河北大学生命科学学院生物芯片研究室,河北省保定市,071002
2. City of Hope National Medical Center,Duarte,California 91010,United States |
| |
| 摘 要： | 目的:检测癌-睾丸抗原GAGE-7的自身抗体在食管鳞状细胞癌患者和正常人血清中表达的差异,评价GAGE家族作为肿瘤诊断标志物的可行性.方法:在原核细菌中表达并纯化重组GAGE-7蛋白.采用ELISA检测GAGE-7自身抗体在105份食管鳞状细胞癌患者血清和86份正常人血清中的表达水平.同时检测各血清中总IgG的表达量,计算GAGE-7自身抗体在总IgG中所占比例的差异.结果:血清中GAGE-7抗体在食管鳞状细胞癌中的含量与正常组比较差异显著(P<0.001),而GAGE-7抗体与总IgG比例,患者组明显高于正常组,逻辑回归模型分析c值为0.717,敏感性和特异性分别达到0.72和0.62,且差异显著(P<0.0001).结论:癌-睾丸抗原GAGE-7的自身抗体在食管鳞状细胞癌血清中有较高表达,且与正常组比较有显著差异,其表达特性可被看做是较为理想的癌症检测标志物.
|
| 关 键 词： | 食管鳞状细胞癌 GAGE家族 自身抗体 血清 |
Detection of serum autoantibody against GAGE-7 in patients with esophageal squamous cell carcinoma |
| |
| Hao Wang,Li Zhong,Jia-Xin Xu,Xiao-Gang Zhang,Jian-Fei Wang. Detection of serum autoantibody against GAGE-7 in patients with esophageal squamous cell carcinoma[J]. World Chinese Journal of Digestology, 2010, 18(10) |
| |
| Authors: | Hao Wang Li Zhong Jia-Xin Xu Xiao-Gang Zhang Jian-Fei Wang |
| |
| Affiliation: | Hao Wang,Li Zhong,Jia-Xin Xu,Xiao-Gang Zhang,Jian-Fei Wang,Biochip Laboratory,College of Life Sciences,Hebei University,Baoding 071002,Hebei Province,China City of Hope National Medical Center,Duarte,California 91010,United States |
| |
| Abstract: | AIM:To compare the level of serum autoantibody against cancertestis antigens GAGE-7 between patients with esophageal squamous cell carcinoma and normal controls and to evaluate the possibility of using GAGE family members as diagnostic tumor markers.METHODS:Recombinant GAGE-7 protein was expressed and purified.The level of serum autoantibody against GAGE-7 was detected in patients with esophageal squamous cell carcinoma serum and normal controls by enzyme-linked immunosorbent assay (ELISA).Meanwhile,total s... |
| |
| Keywords: | IgG |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
