| 裸鼠转基因结肠癌肝转移模型的建立 |
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| 引用本文： | 黎成金,张宝明,王烈,涂小煌,王羊.裸鼠转基因结肠癌肝转移模型的建立[J].肿瘤防治研究,2010,37(2):150-152. |
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| 作者姓名： | 黎成金 张宝明 王烈 涂小煌 王羊 |
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| 作者单位： | 1.350025南京军区福州总医院普通外科研究所；2.陕西省咸阳市中心医院普外科 |
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| 摘 要： | 目的 探讨裸鼠转基因结肠癌肝转移模型的建立方法。方法 应用体外转染法和体内转染法建立裸鼠转基因结肠癌肝转移的动物模型。观察肝脏转移率和自然生存期；瘤组织作病理切片；实时荧光定量（Real-Time RT-PCR）检测CD基因表达，Western blot检测目的基因蛋白表达。结果 体外转染法与体内转染法裸鼠肝脏转移率、自然生存期比较差异无统计学意义100％(15/15),100％(15/15); (40.60±2.2297)d, (41.73±2.4919)d; P>0.05]。实时荧光定量PCR进行相对定量比较，体内转染法CD基因的表达活性低于体外转染法（P＜0.05，t=2.758）。体内转染法瘤组织CD基因蛋白表达低于体外转染法。结论 体外转染法建立裸鼠转基因结肠癌肝转移模型，目的基因在瘤组织中能更稳定、持续表达。
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| 关 键 词： | 结肠肿瘤 肝转移 基因 模型 裸鼠 |
| 收稿时间： | 2008-9-4 |
| 修稿时间： | 2009-2-19 |
Nude Mice Transgenic Hepatic Metastasis Model of Colon Cancer |
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| LI Cheng-jin,ZHANG Bao-ming,WANG Lie,TU Xiao-huang,WANG Yang.Nude Mice Transgenic Hepatic Metastasis Model of Colon Cancer[J].Cancer Research on Prevention and Treatment,2010,37(2):150-152. |
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| Authors: | LI Cheng-jin ZHANG Bao-ming WANG Lie TU Xiao-huang WANG Yang |
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| Institution: | 1.Research Institute of General Surgery Fuzhou General Hospital of Nanjing Military Command Fuzhou 350025，China； 2.Department of General Surgery, Xianyang Central Hospital |
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| Abstract: | Objective To explore the method to establish transgenic animal model for liver metastasis of colon cancer. Methods After establishing transgenic models for liver metastasis of human colon cancer Lovo cell which transfeced with CD gene in nude mice in vitro and in vivo,the rate of liver metastasis and the life span were detected between two groups. Pathological features were observed by HE stain. The RNA and protein expression of CD gene was analyzed by Real-time quantitative RT-PCR and Western-blot. Results... |
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| Keywords: | Colon neoplasm Liver metastasis Gene Models Nude mice |
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