| 乳腺癌耐药蛋白重组腺病毒干扰载体构建及其干扰作用的观察 |
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| 引用本文： | 唐勇,刘传亮,窦建新,蔡晓珊,刘静,陈伟娟.乳腺癌耐药蛋白重组腺病毒干扰载体构建及其干扰作用的观察[J].中华肿瘤防治杂志,2008,15(18):1374-1376. |
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| 作者姓名： | 唐勇 刘传亮 窦建新 蔡晓珊 刘静 陈伟娟 |
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| 作者单位： | 1. 潍坊市人民医院保健三科,山东,潍坊,261041
2. 潍坊医学院病理学教研室,山东,潍坊,261053 |
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| 摘 要： | 目的:观察腺病毒加载的RNAi对乳腺癌耐药蛋白(BCRP)介导的多药耐药的逆转。方法:构建干扰BCRP mRNA的腺病毒载体,采用细胞毒性实验和外排实验检测pAd-BCRP感染后,耐药细胞后的逆转情况。结果:pAd-BCRP可将B37/MX细胞对米托蒽醌的耐药指数从978降低至75(92.3%逆转)。外排实验结果显示,感染pAd-BCRP和未感染的B37/MX细胞外排1 h后细胞内的米托蒽醌的荧光强度分别为56.4和12.8。结论:pAd-BCRP能显著降低B37/MX细胞中BCRP的表达并抑制BCRP的功能。
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| 关 键 词： | 乳腺肿瘤 药物耐受性 肿瘤蛋白质类 RNA干扰 腺病毒科 遗传载体 |
Construction of RNAi recombinated adenovirus targeting BCRP and its inhibitive effects on BCRP |
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| TANG Yong,LIU Chuan-liang,DOU Jian-xin,CAI Xiao-shan,LIU Jing,CHEN Wei-juan.Construction of RNAi recombinated adenovirus targeting BCRP and its inhibitive effects on BCRP[J].Chinese Journal of Cancer Prevention and Treatment,2008,15(18):1374-1376. |
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| Authors: | TANG Yong LIU Chuan-liang DOU Jian-xin CAI Xiao-shan LIU Jing CHEN Wei-juan |
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| Abstract: | OBJECTIVE:To observe the reversing effects of RNAi deliveried by adenovirus on BCRP mediated multidrug resistance.METHODS: RNAi adenovirus targeting breast cancer resistant protein(BCRP) was constructed.The reversal of BCRP was detected by cytotoxicity assays and efflux assays after infection with pAd-BCRP.RESULTS: The multidrug resistance index decreased from 987 to 75(92.3% reverse).The results of efflux assay showed that,after an hour's discharge,the fluorescence intensities were 56.4 and 12.8 in the B37/MX cells infected with adenovirus and without the infection,respectivly.CONCLUSION: RNAi deliveried by adenovrius can decrease the expression of BCRP and reverse MDR mediated by BCRP. |
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| Keywords: | breast neoplasms drug tolerance neoplasm proteins RNA interference adenoviridae genetic vectors |
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