30例女性SLE患者淋巴细胞培养上清液中IL-2、TNF-α、IFN-γ、IL-4水平测定

目的:通过测定系统性红斑狼疮(SLE)外周血淋巴细胞上清液(PBLS)中细胞因子(IL-2、TNF-a、IFN-g、IL-4)水平,探讨系统性红斑狼疮的发病机制,机体内免疫系统失衡的机理,及上述几种细胞因子对SLE的诊断价值。方法:酶联免疫吸附法(ELISA)法检测淋巴细胞培养上清液(PHA刺激前后)中IL-2、TNF-α、IFN-γ及IL-4水平。结果:SLE患者淋巴细胞培养上清液中IL-2水平与正常对照组比较明显降低;TNF-α水平与正常对照组比较略有降低;IFN-γ水平与正常对照组比较明显降低;IL-4水平与正常对照组比较轻度升高。结论:SLE患者体内存在Th1/Th2细胞因子的比例失衡,即产生Th1型细胞因子减低,产生Th2型细胞因子的细胞增加,Th1/Th2比例向Th2极性偏移。

Levels of the cytokine(IL-2,TNF-α,IFN-γand IL-4)in the supernatant of cultivating lymphocyte among 30 cases female patients with SLE

Objective:Determining the levels of the cytokine (IL-2 , TNF-α, IFN-γ and IL-4) in peripheral blood lymphocytes supernatant among the patients with SLE, in order to investigate the pathogenesis of SLE and the mechanism of imbalance in immune system, and identify their diagnostic values.Methods:The levels of the cytokine in lymphocyte culture supernatant were detected by enzyme-linked immunosorbent assay, and PHA stimulation from beginning to the end.Results:Compared with the normal control, the levels of IL-2 and IFN-γ in the lymphocyte culture supernatant of the patients were significantly decreased, TNF-α was slightly decreased, and IL-4 was slightly increased.Conclusion:The ratio of Th1/Th2 cytokine imbalance exists in SLE patients, which reduces Th1-type cytokines and increases Th2-type cytokines, the ratio of Th1/Th2 deflects to Th2.