大鼠移植心脏的细胞凋亡及其与急性排斥反应的关系

目的 观察移植心脏的细胞凋亡现象及其与急性排斥反应的关系。方法 建立大鼠异位心脏移植模型，用ＨＥ梁色和原位末端标记（ＴＵＮＥＬ）技术检测移植心脏切片，进行排斥反应的病理分级，计算凋亡指数（ＡＩ）。结果 发生凋亡的细胞主要是心肌细胞，在各级排斥反应中均可见凋亡细胞存在，且ＡＩ与急性排斥发生的分级成正相关；各级的ＡＩ与０级（无排斥反应）比较，差异均有显著性。结论 细胞凋亡与移植心脏急性排斥反应的严重程

Apoptosis in cardiac ailograft and its relation with acute rejection in rats

Objectives To determine whether apoptotic cell death is involved in rat cardiac allograft rejection and investigate the relevance of apoptosis with acute rejection and its implication. Methods Groups of Wistar rats underwent heterotopic heart transplantation from allogeneic SD or syngeneic Wistar rats. The cardiac grafts were harvested at 1, 3, 5, or 7 days after transplantation and underwent the detection of apoptotic cell death using in situ terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labeling (TUNEL). Histopathological rejection grade and apoptotic index (AI) were analyzed. Results The incidence of apoptotic cells was increased steadily over time in allografts, in contrast to syngeneic grafts. The apoptotic cells in allografts were mainly cardiac myocytes and few infiltrating lymphocytes. The AI of rejection grade 1, 2, 3 and 4 was significantly higher than that of rejection grade 0 ( P < 0.01 ). Conclusions TUNEL can display apoptosis of single cell in situ. Apoptosis is an important mechanism of tissue injury in acute cardiac allograft rejection in rats. Myocyte apoptosis can be used as a valuable index to estimate the injury of grafts and monitor acute rejection.