庆大霉素诱导大鼠肾性贫血模型的建立及基因重组人红细胞生成素的药效学研究

用庆大霉素诱导建立大鼠肾性贫血模型并以此观察本所研制的基因组人红细胞生成素（ｒｈＥＰＯ）对肾性贫血的治疗效应。连续１６ｄ给大鼠皮下注射庆大霉素，病理学研究证实大鼠肾脏组织学结构已发生破坏；实验室指标显示血清尿素氮、肌酐升高，红系各指标显著降低；ＥＬＩＳＡ法不能测出血清中的红细胞生成素（ＥＰＯ），表明大鼠已发生功能衰竭和肾性贫血。经连续５ｄ皮下注射ｒｈＥＰＯ，血清ＥＰＯ水平上升，网织红细胞（Ｒｃ）、红细胞计数、血红蛋白和红细胞比容等指标迅速好转，其中以Ｒｃ最为敏感，提示经ＥＰＯ刺激后人鼠骨髓造血功能增强，其效应强弱与ｒｈＥＰＯ用量相关。实验结果证实ｒｈＥＰＯ对肾性贫血有较好的疗效。

Effect of recombinant human erythropoietin on renal failure models induced by gentamycin in rats

It was demonstrated pathologically that the histological structures of kidneys were impaired after subcutaneous injection of gentamycin for 16 consecutive days in rats.Laboratory parameters showed that serum BUN and Cr elevated,while the indices of the erythrogenic line reduced markedly,and erythropoietin (EPO) was not detecatable by ELISA.When the rats received recombinant human erythropoietin (rhEPO) for 5 d,the level of EPO in serum went up,and the indices of reticulocyte,RBC,Hb and hematocrit improved rapidly, of which the response of reticulocyte was most sensitive.The results suggest that the erythrogenic function of the bone marrow in rats was enhanced after the stimulation of rhEPO and the effect of rhEPO was dose dependent.