| 头孢拉定、头孢氨苄、头孢羟氨苄在反相高效液相色谱中保留行为的研究 |
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| 引用本文： | 胡昌勤,李勇军,徐漫江,金少鸿. 头孢拉定、头孢氨苄、头孢羟氨苄在反相高效液相色谱中保留行为的研究[J]. 中国抗生素杂志, 1993, 0(6) |
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| 作者姓名： | 胡昌勤 李勇军 徐漫江 金少鸿 |
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| 作者单位： | 中国药品生物制品检定所 北京100050(胡昌勤，李勇军，徐漫江)，中国药品生物制品检定所 北京100050(金少鸿) |
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| 摘 要： | 本文系统地报道了头孢拉定、头孢氨苄、头孢羟氨苄在ODS C_(18)色谱柱中的保留行为;头孢拉定的容量因子(K’)最大,头孢氨苄的容量因子居中,头孢羟氨苄的容量因子最小;色谱过程中系统的焓变(△H)<0,logK’和柱温(T)呈线性关系;在甲醇和乙腈两种强溶剂系统中,实验范围内logk’和流动相中强溶剂浓度呈线性关系,乙腈对logk’的影响较甲醇大;流动相的pH通过影响头孢菌素的离子化程度改变头孢菌素的保留行为。
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| 关 键 词： | 头孢拉定 头孢氨苄 头孢羟氨苄 高效液相色谱 |
STUDY ON THE RETENTION BEHAVIOUR OF CEPHRADINE, CEPHALEXSN AND CEFADROXIL IN REVERSED-PHASE HPLC |
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| Hu Chang-qin,Li Yong-jun,Xu Man-Jiang and Jin Shao-hong. STUDY ON THE RETENTION BEHAVIOUR OF CEPHRADINE, CEPHALEXSN AND CEFADROXIL IN REVERSED-PHASE HPLC[J]. Chinese Journal of Antibiotics, 1993, 0(6) |
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| Authors: | Hu Chang-qin Li Yong-jun Xu Man-Jiang Jin Shao-hong |
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| Abstract: | The retention behaviour of Cephradine, Cephalexin and Cefadroxil on ODS C_(18) Column have been studied.The relationship between capacity factor (k') and temperature of the column conformed to the equation of logk' = a b/T and the influence of concentration of strong solvent in binary mobile phase on the k' in the experiments based on the retention equation of logk' = a cC_B The pH value of the mobile phase also effected a change in the k' values by influencing upon the ion-ization of the Cephalosporins. |
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| Keywords: | Cephradine Cephalexin Cefadroxil HPLC |
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