血清及胆汁SIgA夹心EIA测定法

本SIgA夹心酶免疫(EIA)定量分析法是以抗人分泌成分(SC)抗体包被固相载体、辣根过氧化物酶(HRP)标记抗人IgA抗体的Fab′片断作为酶标抗体。本方法灵敏度达O.625ng/孔,特异性强,批内变异系数和批间变异系数分别为5.8～9.3％和6.2～9.2％,回收率为99.6～108.7％,与相应的RIA法高度相关(r=0.961),标本用量甚微,2～4μl血清就可测定。用本方法测定117例正常成人血清,SIgA正常值为2.98～18.15μg/ml(8.04±3.60μg/mld);16份“T”管胆汁标本测定结果为40.8±25.0μg/ml。

SENSITIVE,MICROSCALE SANDWICH ENZYME IMMUNOASSAY FOR SIgA IN HUMAN SERUM AND BILE

This sandwich enzyme immunoassay (EIA) for specific quantitation of secretory immunoglobulin A (SIgA) in human serum and bile involved use of polystyrene plate coated with IgG of rabbit anti-human secretory component (SC) as solid phase, and (anti-IgA) Fab'-HRP conjugate. The detection limit of SIgA was 0.625ng/well without the interference by IgA and SC; coefficients of variation between-run and within-run were 6.2～9.2% and 5.8～9.3% respectively; recovery rates of SIgA in samples were 99.6～108.7%. SIgA values of 30 serum samples estimated by the EIA correlate well with those obtained by a EIA method (r = 0.961). 2～4 ul sample was enough for measurement. By using this assay, the concentrations of SIgA in 117 normal human sera and 16 T-tube drainage bile samples were determined.