迷走神经参与胃伤害性信息向下丘脑的传递

目的　 研究迷走神经是否参与胃伤害性信息向下丘脑室旁核的传递。 方法　 检测下列条件下c Fos蛋白在孤束核及下丘脑室旁核的表达 :①胃内注入福尔马林引起伤害性刺激 ;②福尔马林刺激结合双侧膈下迷走神经切断术。结果　胃内注入福尔马林引起的伤害性刺激可以诱导c Fos蛋白在孤束核和下丘脑室旁核等脑区的表达 ,但在胸段脊髓的I,V ,VII和X层无明显表达。胃内注入生理盐水的对照组则仅有极少量的表达 ,双侧膈下迷走神经切断术可以减少c Fos蛋白在这些部位的表达。 结论　 该研究结果表明 ,迷走神经参与了胃内脏伤害性信息向孤束核及下丘脑室旁核的传递

Transmission of gastric noxious signals to hypothalamus mediated by vagus nerve

Objective To determine whether the vagus nerve mediate the transmission of gastric noxious signals to the paraventricular nucleus of hypothalamus (PVN). Methods Twenty-four SD rats were divided into 4 groups randomly: group 1( n =6) received saline infusion into stomach as control to group 2, group 2( n =6) received formalin infusion into stomach, group 3 ( n =6) received sham operation (opening peritoneum) as control to group 4, group 4( n =6) received bilateral subdiaphragmatic vagotomy plus formalin infusion into stomach. c-Fos like immunoreactivity (c-FLI)-positive neurons was observed in nucleus tractus solitarius (NTS) and PVN of 4 groups. Results Noxious stimulation of formalin infusion into the stomach induced c-Fos protein expression in NTS and PVN, but not the thoraco-lumbar spinal cord, while almost no c-Fos labeling was detected in controls, and bilateral subdiaphragmatic vagotomy reduced the c-Fos expression significantly. Conclusion The blockage of c-Fos expression in NTS and PVN after bilateral subdiaphragmatic vagotomy suggested the involvment of vagus nerve in the transmission of gastric noxious signals to the NTS and PVN.