白介素10对大鼠缺血梗死灶周围脑组织Bcl-2和Bax表达的作用研究

目的 探讨白介素10对大鼠脑缺血梗死灶周围Bcl-2和Bax表达的作用.方法 18只SD大鼠随机分为假手术组(Sham组)、脑缺血组(MCAO组)、脑缺血 溶剂对照组(Vehicle组)和脑缺血 IL-10干预组(IL-10组),免疫组化和RT-PCR法观察术后24h梗死灶周围脑组织Bcl-2和Bax表达水平的变化.结果与Sham相比,MCAO组梗死灶周围脑组织Bcl-2和Bax的表达显著上调,Bcl-2/Bax比值显著下降;与Vehicle组比,IL-10组Bcl-2的表达显著上调,Bax表达显著下调,Bcl-2/Bax比值显著增加.结论 IL-10与脑缺血梗死灶周围Bcl-2表达上调,Bax表达下调,Bcl-2/Bax比值增加有关,提示IL-10可能与抑制梗死灶周围脑组织神经细胞凋亡有关.

Effects of interleukin-10 on Bcl-2 and Bax expressions in cerebral ischemia of rats

Objective To investigate the effects of interleukin-10 on Bcl-2 and Bax expressions in peri-infarct zone of cerebral ischemic rats. Methods Forty eight male adult Sprague-Dawley rats were randomly assigned into four groups: Sham operation group, middle cerebral artery occlusion group (MCAO group), MCAO plus vehicle adminerstration group (Vehicle group), and MCAO plus interleukin-10 administration group (IL-10 group). Twenty four hours after operation, rats were sacrificed by decapitation, and the level of Bcl-2 and Bax expression in peri-infarct cerebral tissue were measured by Immunohistochemical (ICH) and RT-PCR. Results In rats of MCAO group, the expressions of Bcl-2 and Bax and the rate of Bcl-2/Bax were decreased significantly, compared with Sham group (P<0.01). In rats of IL-10 group, the Bcl-2 expression was upregulated (P<0.05), the Bax expression was downregulated (P<0.01), and the rate of Bcl-2/Bax was increased (P<0.01), compared with vehicle group. Conclusion Interleukin-10 is significantly related to the upregulation of Bcl-2 expression, the downregulation of Bax expression, and the increase of the ratio of Bcl-2/Bax in peri-infarct zone of cerebral ischemia, which suggest that interleukin-10 could be related with the inhibitory effects on ischemic neurocyte apoptosis.