黄芪注射液对人乳腺癌细胞中VEGF和CXCR4表达的影响

目的：研究黄芪对人乳腺癌细胞中MDA-MB-231系血管内皮生长因子（VEGF）及其受体（flk-1）和血小板源性因子受体-4（CXCR4）表达的影响。方法：在无酚红DMEM培养的人乳腺癌细胞中加0.5、1.0、2.0 g/L黄芪处理,对照组（Con）加等体积的蒸馏水。收集培养48 h的细胞,用免疫细胞化学和免疫印迹检测乳腺癌细胞中VEGF、flk-1和CXCR4蛋白的表达。结果：与Con组相比,黄芪组乳腺癌细胞中的VEGF、flk-1和CXCR4蛋白表达均下降,且高浓度组中表达明显降低。结论：高浓度黄芪能下调乳腺癌细胞中VEGF、flk-1和CXCR4蛋白的表达量,由此可能会减少肿瘤血管生成和降低肿瘤侵袭。

Effects of astragalus mongholicus on the expressions of VEGF and CXCR4 in human breast cancer cells

Objective：To investigate the effects of astragalus mongholicus on the expressions of vascular endothelial growth factor（VEGF）,VEGF receptor（flk-1）,and CX chemokine receptor type 4（CXCR4） in MDA-MB-231 breast cancer cells.Methods：MDA-MB-231 breast cancer cells were cultured in phenol red-free DMEM and were treated with 0.5g/L,1.0g/L,2.0g/L astragalus mongholicus.The control group was treated with isovolumic distilled water.Western blotting and immunocytochemistry methods were used to detect the expressions of VEGF,CXCR4 and flk-1.Results：The expressions of VEGF,CXCR4 and flk-1 were lower than those of the control.There was significant difference between the high concentration group and the control.Conclusion：The expressions of VEGF,CXCR4 and flk-1 are down-regulated when breast cancer cells were treated with astragalus mongholicus at high concentration,therefore the angiogenesis and invasion of breast tumors maybe reduced.