COPD大鼠模型肺密度与血清 HSP27的相关性研究

目的：探讨 COPD 大鼠模型肺密度与血清热休克蛋白27(heat shock proteins 27, HSP27)的相关性。方法将40只Wistar大鼠随机分为5组(G1~G5),每组再分2亚组(COPD模型组及正常对照组),采用烟熏加气管内注入脂多糖的方法制造 COPD 大鼠模型。用 HE 染色法评价大鼠气道壁病理变化；采用 ELISA法检测血清中 HSP27的表达水平；并对每组大鼠肺部行128层螺旋CT扫描,测量其平均肺密度。COPD模型组肺密度及血清 HSP27表达水平的各组间比较采用方差分析(LSD-t 检验),肺密度与血清 HSP27表达水平之间的关系采用 Pearson 相关性分析。结果 COPD模型组大鼠气道的炎性改变随时间不断加重。模型组大鼠的平均肺密度早期稍下降,第3、4周有所升高,第6周明显下降,整体呈下降趋势；COPD模型组肺密度组间比较差异有统计学意义(F=23.516,P<0.01)；正常对照组肺密度变化不明显。COPD模型组血清 HSP27的表达水平高于正常对照组,HSP27的表达呈轻微上升趋势,G4、G5组之间差异有统计学意义(P <0.05)。COPD模型组血清 HSP27的表达水平与肺密度呈低度负相关(r=-0.421,P <0.05),随肺密度的减低而升高。结论血清 HSP27表达水平的改变一定程度上反映了肺气肿的严重程度,可以作为评估 COPD肺组织受损严重程度的一个指标。

Association between the pulmonary density and serum HSP27 in the rat model of COPD

Objective To investigate the correlation between the pulmonary density and serum HSP27 in the rat model of COPD.Methods Forty Wistar rats were divided into 5 groups randomly(G1-G5).Each group consisted of two subgroups:COPD model group and normal control group.The rat model of COPD was made by smoking and lipopolysaccharide inj ected into the trachea.To evaluate the change of pathology in rats lung tissue by HE,and detect the levels of HSP27 in serum by ELISA.Each group of rat was performed by 128 slice spiral CT scanning,and the average lung density was measured.Analysis of variance(LSD-t test)was used to compare the lung density and the levels of serum HSP27 in the COPD model group,and the relationship between the lung density and HSP27 in the serum was analyzed by Pearson correlation.Results The inflammatory changes of airway in COPD model group were increased with time.The mean lung density of model group slightly decreased early.It was increased in third and four weeks while significant decreased in sixth weeks,and presented a trend of rising on the whole.The lung density of COPD model group was significantly different between groups,and the difference was statistical significance(F=23.516,P <0.01).The change of lung density was not obvious in normal control group.Compared with control group,the levels of serum HSP27 was increased in COPD model groups.The levels of HSP27 had a slightly rising tend,there were statistical significance between fourth groups and fifth groups(P<0.05).The levels of HSP27 was negatively correlated with the lung density in the COPD model group (r =-0.421,P <0.05),which was increased with the decrease of lung density.Conclusions The change of serum HSP27 reflects the severity of pulmonary emphysema,and can be used as a marker to assess the severity of COPD and lung inj ury.