黄芪甲苷对大鼠肺缺血再灌注后氧化应激损伤的影响

目的：研究黄芪甲苷对大鼠肺缺血再灌注损伤后氧化应激的影响。方法将50只实验用大鼠随机分为假手术组、模型组和黄芪甲苷(20、40、80 mg/kg)组,每组10只。采用夹闭左肺门45 min后松夹的方法制备肺缺血再灌注损伤大鼠模型,各组分别于术前30 min腹腔注射给药。再灌注2h后,测定各组大鼠肺组织湿/干重比,测定肺组织中抗氧化酶(超氧化物歧化酶、谷胱甘肽过氧化物酶、过氧化氢酶)活性,测定血清中总抗氧化能力、髓过氧化物酶活性和丙二醛含量,通过HE染色法观察肺组织形态结构变化,TUNEL 法观察肺组织细胞凋亡状况并计算凋亡指数,采用Western blot法测定肺组织中核转录因子κB蛋白表达并进行半定量分析。结果与模型组比较,黄芪甲苷(40、80 mg/kg)组大鼠肺组织湿/干重比显著降低,肺组织中超氧化物歧化酶、谷胱甘肽过氧化物酶、过氧化氢酶活性显著升高,血清中总抗氧化能力升高,髓过氧化物酶活性、丙二醛含量降低,肺组织形态结构变化和肺细胞病变显著减轻,肺细胞凋亡状况明显改善,肺细胞凋亡指数降低,肺组织中核转录因子κB蛋白表达量降低,差异均有统计学意义(P<0.05或P<0.01)。结论黄芪甲苷具有抑制肺缺血再灌注损伤大鼠氧化应激的作用,作用机制可能与黄芪甲苷能够改善肺组织和血清中抗氧化酶活性、提高氧自由基清除能力以及下调核转录因子κB蛋白表达、抑制肺细胞凋亡有关。

Effects of astragalosides against oxidative stress in rats with pulmonary ischemia-reperfusion injury

Objective To investigate the effects of astragalosides against oxidative stress in rats with pulmonary ischemia-reperfusion inj ury.Methods Fifty experimental rats were randomly divided into five groups:sham operation group,model group and astragalosides(20,40,80 mg/kg)groups(n=10). The pulmonary ischemia-reperfusion model rats were made by clipping left lung hilum for 45 min.The drugs were given by intraperitoneal injection at 30 min before operation.At 2 h after reperfusion,the wet/dry ratio of lung tissue was examined,the activities of superoxide dismutase (SOD),glutathione peroxidase (GSH-Px),catalase (CAT) in lung tissue and myeloperoxidase (MPO) in serum were determined,the level of total antioxidant capacity (T-AOC) and the content of malondialdehyde (MDA) in serum were determined,the histopathological changes and cell apoptosis of lung tissue were observed, and the apoptosis index was analyzed,the expression of nuclear factor-κB (NF-κB) in lung tissue was determined by Western blot and semi-quantitative analyzed.Results Compared with model group,the lung wet/dry ratio was significantly decreased,the activities of SOD,GSH-Px,CAT in lung tissue were increased,the level of T-AOC in serum was increased,the activity of MPO and the content of MDA were decreased,the histopathological changes and the lung cell apoptosis were significantly improved,the apoptosis index was decreased,the expression of NF-κB protein in lung tissue was decreased in astragalosides(40,80 mg/kg) groups (P <0.05 or P <0.01).Conclusions Astragalosides has inhibitive effects against oxidative stress in rats with pulmonary ischemia-reperfusion inj ury,which is perhaps related to improving antioxidant ability,improving the free radical scavenging ability and down-regulating the expression of NF-κB protein,depressing the lung cell apoptosis.