溶藻弧菌感染小鼠导致的炎性因子变化

目的探讨溶藻弧菌感染小鼠后引起的炎性因子水平变化及意义。方法建立溶藻弧菌ATCC17749T腹腔注射感染BALB/c小鼠动物模型,进行血液学和肝功能指标检测以及肝脏病理学检查,采用炎性因子抗体芯片进行炎性因子检测。结果溶藻弧菌ATCC17749T感染BALB/c小鼠的半数致死量为1×109 CFU。感染组小鼠血液白细胞和血小板数目均降低,差异有统计学意义(P0.05);红细胞和血红蛋白量、丙氨酸氨基转移酶、天门冬氨酸氨基转移酶水平均升高,差异有统计学意义(P0.05);病理学观察发现,溶藻弧菌感染可引起小鼠肝、肺严重损伤;炎性因子检测筛选出20种显著变化的炎性因子,其中细胞因子信号值大于200,倍数改变大于10的有8种炎性因子,分别为KC、IL-6、RNATES、IL-12、Eotaxin、G-CSF、MIP-1α、Mig。结论该研究所筛选出的溶藻弧菌感染小鼠后变化的炎性因子为溶藻弧菌炎症机制的研究提供了理论依据。

The changes of inflammatory factors in mice infected with Vibrio alginolyticus

Objective To investigate the changes of inflammatory factors in mice infected with Vibrio alginolyticus .Methods Established a V .alginolyticus ATCC17749 T‐infected mouse model through intraperitoneal injection ,then performed the median le‐thal dose experiment ,the detection of hematological and liver function indicators ,histopathologic evaluation and inflammatory cyto‐kine detection .Results The median lethal dose of V .alginolyticus ATCC17749T was 1 × 109 CFU by intraperitoneal injection .The dose of 1 × 109 CFU per mouse was adopted in the following study .WBC and platelet numbers significantly decreased after infection (P<0 .05) ,RBC number and hemoglobin content significantly increased(P<0 .05) and ALT ,AST significantly increased after V . alginolyticus infection(P<0 .05);light microscopy observation revealed that ,hepatic cell and pulmonary tissue suffered serious inju‐ry after infection.Through the detection of antibody array ,the levels of 20 inflammatory factors were found changed significantly . 8 of them including KC ,IL‐6 ,RNATES ,IL‐12 ,Eotaxin ,G‐CSF ,MIP‐1a ,Mig ,the expression of which increased more than 10 times .Conclusion The inflammatory factors analysed in the paper provide a theoretical basis for the study of the inflammatory mechanism of Vibrio alginolyticus infection .