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| MICA新等位基因MICA*002:04的DNA序列鉴定及分析 | |
| 引用本文： | 谭茵,黄颖烽,徐秀章,叶欣,魏亚明,古维立.MICA新等位基因MICA*002:04的DNA序列鉴定及分析[J].中国免疫学杂志,2011,27(5):434-437,445. |
| 作者姓名： | 谭茵 黄颖烽 徐秀章 叶欣 魏亚明 古维立 |
| 作者单位： | 1. 广州医学院基础学院,广州,510182 2. 广州医学院附属广州市第一人民医院,广州,510180 3. 广州血液中心,广州,510095 |
| 基金项目： | 广东省自然科学基金，广州市医药卫生科技一般引导项目 |
| 摘 要： | 目的:直接测序法及外显子3 DNA片段克隆测序定确认MICA新的等位基因MICA*002:04。方法:用组特异性引物分别扩增MICA基因,采用Sequence Base Typing(SBT)法分型技术及PCR片段克隆测序法对MICA多态性基因的外显子2、3、4、5进行双向测序。结果:发现1个与MICA*002:01序列相近的新等位基因,在外显子3上有1个碱基位置与国际通用MICA数据库不相符。该基因与MICA*002:01相比在外显子3的碱基位置486出现突变(C→A),密码子位置20由GCC→GCA,相应编码氨基酸是同义突变。结论:DNA测序结果表明该基因序列为新的MICA等位基因,已提交GenBank,于2010年9月被世界卫生组织HLA因子命名委员会正式命名为MICA*002:04。 |
| 关 键 词： | DNA测序分型 MICA 新等位基因MICA*002 |
Identification of a novel MICA allele MICA*002:04 by sequencing based typing and clone sequencing |
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| TAN Yin,HUANG Ying-Feng,XU Xiu-Zhang,YE Xin,WEI Ya-Ming,GU Wei-Li.Identification of a novel MICA allele MICA*002:04 by sequencing based typing and clone sequencing[J].Chinese Journal of Immunology,2011,27(5):434-437,445. | |
| Authors: | TAN Yin HUANG Ying-Feng XU Xiu-Zhang YE Xin WEI Ya-Ming GU Wei-Li |
| Institution: | TAN Yin,HUANG Ying-Feng,XU Xiu-Zhang,YE Xin,WEI Ya-Ming,GU Wei-Li.School of Basic Medicine,Guangzhou Medical College,Guangzhou 510182,China |
| Abstract: | Objective:To identify of a novel MICA allele MICA*002:04 by sequencing Based Typing and Clone Sequenting.Methods:Samples were tested by Sequence Based Typing(SBT) as routine MICA allele typing in a research project supported by Guangdong provincial fund.MICA-PCR DNA fragment was inserted into a pMD-T vector and re-sequenced to confirm the typing results.Results:The DNA sample of a lung cancer patient was found mismatched in MICA gene bank database.Sequence Based Typing(SBT) and clone sequence results showed... |
| Keywords: | MICA |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |