南通市几类人群TTV、HGV、HCMV感染状况的初步调查

目的　调查南通市几类人群中输血传播病毒 (TTV)、庚型肝炎病毒 (HGV)和人巨细胞病毒 (HCMV)的感染状况。方法　选择献血员、非肝病人群、急性病毒性肝炎 (AH)与慢性病毒性肝炎 (CH)患者、肝炎肝硬化 (LC)以及原发性肝癌 (PHC)患者为调查对象。采用半巢式聚合酶链反应检测TTVDNA、巢式聚合酶链反应检测HGVRNA、核酸斑点杂交法检测HCMVDNA。采用微量细胞毒试验方法检测人外周血T细胞亚群。结果　义务献血员、非肝病人群、AH、CH、LC和PHC人群的TTV感染率分别为 9 2 %、2 4 0 %、4 6 6 %、4 1 6 %和 6 3 6 % ,后三种人群的感染率显著高于义务献血员 (P <0 0 1 )。HGV感染率在有偿献血员、AH、CH和LC人群中分别为 2 9%、9 6 %、1 7 6 %和 1 3 8% ,但各组之间无统计学差异 (P >0 0 5 )。HCMV在非肝病人群、AH、CH和LC人群中的感染率各为 2 6 7%、38 9%、6 5 8%和 77 8% ,CH组与LC组的感染率显著高于非肝病人群 (P <0 0 1 )。结论　HGV、TTV、HCMV在南通市人群包括献血员中有一定的感染率 ,在筛选献血员时有必要对此三种病毒进行检测 ,以防止其经输血途径传播

Preliminary Rnvestigation on the Infection Rate of TTV, HGV and HCMV in Some Subpopulations of Nantong City

Objective To investigate the infection status of transfusion transmitted virus(TTV), hepatitis G virus(HGV) and human cytomegalovirus(HCMV) in some subpopulations of Nantong city. Method The investigated objects were selected from the groups of blood donors, normal people, patients with acute or chronic virus hepatitis and those with cirrhosis of liver (LC) or with primary hepatocarcinoma (PHC). The TTV DNA was detected with half nested PCR and the HGV RNA was detected with nested PCR. The HCMV DNA was tested with the dot blot hybridization. The trace cytotoxicity test was adopted to assay the subtypes of T lymphocyte.Results The infection rates of TTV in the groups of volunteer blood donors, patients with acute hepatitis (AH), patients with chronic hepatitis (CH), patient with LC and patient with PHC were 9 2%, 24 0%, 46 6%, 41 6% and 63 6% respectively. The infection rates in three groups of patient with CH, LC and PHC were all significantly higher than that in the group of volunteer blood donors. The infection rates of HGV in the group of blood transfusion recepients, patients with AH, patients with CH and patients with LC were 2 9%, 9 6%, 17 6% and 13 8% respectively. The infection rates of HCMV in the groups of normal persons, patients with AH, patients with CH and patients with LC were 26 7%, 38 9%, 65 8% and 77 8% respectively, and the infection rates in groups of patients with CH or LC were both remarkably higher than the group of normal persons.Conclusion Among the population including blood donors of Nantong city, there was a significant percentage of people who are infected by TTV, HGV and HCMV. It is necessary to diagnose of these three viruses when selecting the blood donors to prevent the spreading of these viruses.