Hypothermic Machine Perfusion with Hydrogen Gas Reduces Focal Injury in Rat Livers but Fails to Restore Organ Function |
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| Affiliation: | 1. Gastroenterological Surgery 1, Hokkaido University Graduate School of Medicine, Sapporo, Japan;2. Department of Cell Physiology, Jikei University School of Medicine, Tokyo, Japan;3. Gastroenterological Surgery 1, Hokkaido University Hospital, Sapporo, Japan;4. Department of Transplant Surgery, Hokkaido University Graduate School of Medicine, Sapporo, Japan;5. Division of Organ Transplantation, Hokkaido University Hospital, Sapporo, Japan;1. Third Hospital of Shanxi Medical University, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Taiyuan, China;2. Health Science Center of Xi''an Jiaotong University, The First Affiliated Hospital of Xi''an Jiaotong University, Xi''an, China;1. Department of General Surgery, Ege University Hospital, Izmir, Turkey;2. Department of Pediatry, Ege University Hospital, Izmir, Turkey;3. Department of Gastroenterology, Ege University Hospital, Izmir, Turkey;1. Department of Kidney Center, Sumitomo Hospital, Osaka, Japan;2. Department of Nephrology, Osaka University Graduate School of Medicine, Osaka, Japan;3. Seiwa Hospital, Osaka, Japan;4. Department of Pathology, Sumitomo Hospital, Osaka, Japan;5. Yamaguchi''s Pathology Laboratory, Chiba, Japan;1. Transplant - Urology and Nephrology Department, Pediatric Hospital, Western National Medical Center, Mexican Social Security Institute, Guadalajara, Mexico;2. Research and Teaching Department, Pediatric Hospital, Western National Medical Center, Mexican Social Security Institute, Guadalajara, Mexico;3. and Pediatric Surgery Department, Pediatric Hospital, Western National Medical Center, Mexican Social Security Institute, Guadalajara, Mexico;1. Department of Surgery, Graduate School of Medicine, Tohoku University, Sendai, Miyagi prefecture, Japan;2. Division of Transplantation and Regenerative Medicine, Graduate School of Medicine, Tohoku University, Sendai, Miyagi prefecture, Japan |
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| Abstract: | BackgroundWe have previously reported the efficacy of post-reperfusion H2 gas treatment in cold storage (CS) and subsequent reperfusion of the rat liver. The present study aimed to evaluate the effect of H2 gas treatment during hypothermic machine perfusion (HMP) in rat livers retrieved from donation after circulatory death (DCD) and elucidate the mechanism of action of H2 gas.MethodsLiver grafts were procured from rats after 30 min of cardiopulmonary arrest. The graft was subjected to HMP for 3 hours at 7°C using Belzer MPS with or without dissolved H2 gas. The graft was reperfused using an isolated perfused rat liver apparatus at 37°C for 90 minutes. Perfusion kinetics, liver damage, function, apoptosis, and ultrastructure were evaluated.ResultsPortal venous resistance, bile production, and oxygen consumption rates were identical in the CS, MP, and MP-H2 groups. Liver enzyme leakage was suppressed by MP (vs control), whereas H2 treatment did not show a combination effect. Histopathology revealed poorly stained areas with a structural deformity just below the liver surface in the CS and MP groups, whereas these findings disappeared in the MP-H2 group. The apoptotic index in the CS and MP groups was high but decreased in the MP-H2 group. Mitochondrial cristae were damaged in the CS group but preserved in the MP and MP-H2 groups.ConclusionsIn conclusion, HMP and H2 gas treatment are partly effective in DCD rat livers but insufficient. Hypothermic machine perfusion can improve focal microcirculation and preserve mitochondrial ultrastructure. |
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