Protective effects of Cu/Zn-SOD and Mn-SOD on UVC radiation-induced damage in NIH/3T3 cells and murine skin |
| |
Institution: | 1. College of Life Science, Yangtze University, Jingzhou 434000, PR China;2. Jingzhou Changxin Biotechnology Co., Ltd., Jingzhou 434000, PR China;1. Department of Pathobiology and Immunology, Hebei University of Chinese Medicine, Shijiazhuang 050200, China;2. School of Basic Medical Sciences, Shanxi University of Chinese Medicine, Jinzhong 030619, China;1. Department of Chemical, Biological, Pharmaceutical, and Environmental Sciences, University of Messina, 98166 Messina, Italy;2. Department of Anatomy and Histology, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt;3. Department of Clinical and Experimental Medicine, University of Messina, Padiglione C, A. O. U. Policlinico “G. Martino”, 98124 Messina, Italy;4. Department of Veterinary Sciences, University of Messina, 98168 Messina, Italy;5. Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Sohag University, Sohag 82524, Egypt;6. Institute for Marine Biological Resources and Biotechnology (IRBIM), National Research Council (CNR), Section of Messina, 98100 Messina, Italy;1. Department of Biological Sciences, University of Toronto, Scarborough, Ontario M1C 1A4, Canada;2. Department of Genetics and Development, Krembil Research Institute, Toronto, Ontario M5T 0S8, Canada;3. Department of Human Biology, University of Toronto, Toronto, Ontario M5S 3J6, Canada;4. Department of Pharmacology and Toxicology, University of Toronto, Toronto, Ontario M5S 1A8, Canada;5. Department of Neuroscience, University of Toronto, Scarborough, Ontario M1C 1A4, Canada;6. Department of Biochemistry, University of Toronto, Scarborough, Ontario M1C 1A4, Canada;7. Department of Pharmacy, Silver Oak University, Ahmedabad, Gujarat 382481, India;1. Centro de Microscopia Eletronica, Departamento de Ciencias Biologicas, Universidade Estadual de Santa Cruz, Campus Soane Nazare de Andrade, 45662-900 Ilheus, Brazil;2. Departamento de Clinica e Cirurgia Veterinarias, Escola de Veterinaria, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, Brazil;1. Department of Chemical, Biological, Pharmaceutical, and Environmental Sciences, University of Messina, 98166 Messina, Italy;2. Department of Clinical and Experimental Medicine, University of Messina, Padiglione C, A. O. U. Policlinico “G. Martino”, 98124 Messina, Italy |
| |
Abstract: | Superoxide dismutase (SOD) is an antioxidant enzyme with multiple metal cofactors that can specifically clear reactive oxygen species (ROS), which plays an important role in a variety of ultraviolet-induced lesions. Therefore, SOD has the anti-ultraviolet radiation effect. The objective of this study was to compare the differences in the anti-ultraviolet radiation effect of SOD with distinct metal cofactors: Cu/Zn-SOD and Mn-SOD. SOD was first purified using hydrophobic interaction chromatography and ion-exchange chromatography. Second, the Methylthiazolyldiphenyl-tetrazolium bromide method and cell senescence kits were used to study the protective effect of SOD on ultraviolet-induced cell damage. Finally, the protective effect of SOD on ultraviolet -induced skin damage was histopathologically evaluated, and the expression levels of malondialdehyde (MDA) and matrix metalloproteinases (MMPs) in tissues were detected. The results showed that Cu/Zn-SOD was superior to Mn-SOD in promoting cell proliferation, alleviating cell damage, protecting skin structure, and regulating the expression levels of MDA and MMPs, and it has no side effects. In conclusion, Cu/Zn-SOD had a better anti-ultraviolet radiation effect than Mn-SOD, and it can be used in anti-aging and anti-ultraviolet skin-care products. |
| |
Keywords: | Anti-ultraviolet radiation Superoxide dismutase NIH/3T3 cells Malondialdehyde Matrix metalloproteinase Chromatography |
本文献已被 ScienceDirect 等数据库收录! |
|