Role of MAPK7 in cell proliferation and metastasis in ovarian cancer |
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Authors: | Jinhua Dai Tao Wang Weihua Wang Songhua Zhang Yufeng Liao Jie Chen |
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Affiliation: | 1.Department of Clinical Laboratory, Ningbo No.2 Hospital, Ningbo 315010, Zhejiang, P.R. China;2.Department of Clinical Laboratory, The First Affiliated Hospital of Xiamen University, Fujian Medical University, Xiamen 361003, Fujian, P.R. China;3.Department of Clinical Laboratory, The Affiliated Hospital of Ningbo University, Ningbo 315020, Zhejiang, P.R. China;4.Department of Gynaecology, Ningbo No.2 Hospital, Ningbo 315010, Zhejiang, P.R. China |
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Abstract: | Aim: To investigate the effect of mitogen-activated protein kinase 7 (MAPK7) in ovarian cancer metastasis and to explore its potential mechanism. Methods: pcDNA-MAPK7 and siRNA-MAPK7 vectors were transfected into the human ovarian cell line OVCAR-3 based on gene silencing and overexpression methods. Effects of MAPK7 overexpression and silencing on OVCAR-3 cells proliferation, cell invasion, and migration were analyzed using the MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide) assay, Matrigel methods, and Markered methods respectively. In addition, effect of MAPK7 expression on extracellular matrix (ECM) associated protein was detected using Western blot. Results: Compared with the controls, MAPK7 was up-regulated when cells were transfected with pcDNA-MAPK7 plasma, as well as MAPK7 was sliced when cells were transfected with siRNA-MAPK7 plasma (P<0.05). Besides, biological function analysis performed that overexpression of MAPK7 significantly increased OVCAR-3 cell proliferation, invasion, and migration (P<0.05), while these effects were inhibited by MAPK7 silencing (P<0.05). Additionally, MAPK7 overexpression increased type II collagen expression (P<0.05). However, there was no significant difference between MAPK7 expression and type I collagen expression (P>0.05). Conclusion: Our data implied the up-regulated MAPK7 might contribute to ovarian cancer metastasis through up-regulating type II collagen expression and then were involved in cell biological processes such as cell proliferation, invasion, and migration. MAPK7 may be a potential therapeutic target in the clinical treatment for ovarian cancer. |
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Keywords: | Ovarian cancer MAPK7 cell proliferation cell invasion metastasis |
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