组织蛋白酶D、EGFR、p16基因在非小细胞肺癌表达的临床意义

目的探讨组织蛋白酶D(Cath-D)、表皮生长因子受体(EGFR)、抑癌基因p16在非小细胞肺癌(NSCLC)表达的意义.方法采用链霉抗生素蛋白-过氧化物酶(SP)快速免疫组化法检测64例肺癌标本3种蛋白的表达.结果肺癌组织Cath-D、EGFR和P16表达的阳性率分别为66%、67%和52%,P16蛋白表达缺失率达48%,Cath-D和EGFR表达与肺癌患者临床分期(P<0.05,P<0.01)、淋巴结转移(P<0.01,P<0.05)密切相关.COX比例风险模型分析和Kaplan-Meier存活曲线分析显示Cath-D和EGFR是影响肺癌患者预后的重要因素(P<0.05).结论在NSCLC中P16、Cath-D和EGFR异常改变的频率很高,在NSCLC的发展中可能起促进作用.Cath-D和EGFR可作为评价NSCLC患者的预后指标.

Expression and clinical significance of cathepsin D,EGFR,P16 in non-small cell lung cancer

Objective To study clinical significance of the expression of cathepsin D(Cath D),epidermal growth factor receptor(EGFR)and P16 in patients with non small cell lung cancer(NSCLC).Methods A rapid immunohistochemical method(streptavidin peroxidase conjugataed method SP)was used to detecting the expression of three proteins the NSCLC patients in 64 cases lung cancer samples.Results The positive expression rate of Cath D,EGFR,P16 was 66%,67% and 52% in the lung cancer tissues,respectively,deletion rate of P16 protein expression was 48.4%.Expression of Cath D,EGFR was significantly related to the stage of the disease ( P <0.05, P <0.01)and lymph node metastasis( P <0.01, P <0.05) in the lung cancer patients.The multivariate analysis using COX hazard model and Kaplan Meier method showed the Cath D and EGFR were the most important influence factors in prognosis of the disease.Conclusion Abnormal expression of all the three factors are very high in the NSCLC patients,they may play promotion roles in development of NSCLC.Cath D and EGFR are the useful parameters to evaluate the prognosis of NSCLC.