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Dynamic cell cycle kinetics in vitro and in vivo in myelodysplastic syndromes with special reference to the influence of hematopoietic growth factors |
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| Authors: | Holm M Hoyer M Jensen I Thomsen M Hokland P |
| Institution: | a Department of Hematology, Immunhæmatologisk Laboratorium, Århus amtssygehus, Tage-Hansens Gade 2, Opgang 4A, DK 8000 Århus, Denmark b Department of Oncology, Onkologisk afdeling, Århus Kommunehospital, Nørrebrogade 44, DK 8000 Århus, Denmark |
| Abstract: | We have investigated the effect of HGF in vivo and in vitro in MDS using a recently developed FCM assay involving the simultaneous measurement of cell surface antigens, DNA content, and BrdUrd or IodUrd incorporation. This allows for the determination of the dynamic cell kinetic parameters: LI, Ts, and Tpot and we observed that in vitro HGF stimulation resulted in a significant decrease in mean Tpot values from 6.6 to 3.5 days. Importantly, we demonstrated that in vivo GM-CSF administration to patients with RAEB resulted in a shortening of Tpot within the 2 first weeks of GM-CSF treatment. |
| Keywords: | Myelodysplastic syndromes Cell cycle kinetics Ts and Tpot In vitro growth factor stimulation GM-CSF administration Heterogeneity |
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