半结扎脑表面动脉构建大鼠局灶性淋巴滞留性脑病动物模型

目的通过半结扎脑表面动脉的方法,构建一种局灶性淋巴滞留性脑病动物模型,观察血管周围间隙(VRS)扩张情况。方法 30只SD雄性大鼠,分为24 h模型组、48 h模型组和假手术对照组(每组各10只)。模型组采用脑表面动脉半结扎术造成局部淋巴回流障碍,术中监测大鼠脑电图变化;假手术组大鼠仅开颅分离脑表面动脉而不行半结扎。假手术组于术后48 h,24 h模型组和48 h模型组分别在术后24和48 h灌注固定并取脑进行冰冻切片,HE染色后在光镜下观察VRS扩张情况,采用Mias2000图像分析系统分别对24 h和48 h模型组以及假手术组的VRS截面积进行定量测量,并进行统计分析。结果脑电图监测显示术后大鼠未出现脑缺血等脑功能障碍改变。半结扎的脑表面动脉支配区域的皮层及皮层下均可见明显扩张的VRS,皮层下白质较多见。扩张的VRS周围脑组织结构疏松,着色浅淡。24 h模型组和48 h模型组半结扎的脑表面动脉支配区域的VRS面积显著大于假手术组(P<0.01),模型组之间无显著差异。结论采用脑表面动脉半结扎术能够有效构建大鼠局灶性淋巴滞留性脑病动物模型,手术创伤小、操作方便,制备的动物模型较稳定,为研究脑内淋巴循环障碍提供了一个较理想的平台。

A rat model of focal lymph encephalopathy established by partial ligation of the cerebral superficial artery

Objective To establish a rat model of focal lymphatic encephalopathy by partial ligation of the cerebral superficial artery for observation of the changes of Virchow-Robin spaces(VRS).Methods Thirty male SD rats were randomized into 3 groups(n=10),including two model groups and a sham-operated group.The rats in the model groups were subjected to partial ligation of the cerebral superficial arteries under EEG monitoring to induce focal lymphatic encephalopathy,and those in the sham-operated group underwent o...