| Abstract: | Although permeation of capillary endothelia by horseradish peroxidase has been related to the presence of narrow channels between cells, junctions between endothelial cells in the aorta contain extensive regions that are occluded by apposition or fusion of the adjacent plasma membranes. Despite this, intravenously infused horseradish peroxidase rapidly appears within both the endothelial cell junctions and the microvesicles and caveoli. This suggests that the enzyme may arrive in the junctional space by some route other than passage between the endothelial cells. One possibility is vesicular transport from the lumen to the junctional cell surface. Alternatively, the occluded regions may represent only a partial permeability barrier, which either incompletely surrounds each cell in the manner of the maculae occludentes of epithelia, or leaves passages open between some cells while completely closing the perimeter of others. The aortic intima also differs from other vessel linings in having a reticular form of basement membrane. The structural role of this reticulum in supporting the endothelium remains undetermined as does its effect on premeability of the intima. |
