膳食纤维对大鼠排锰效果及微量元素影响

目的 探讨膳食纤维的排锰效果及对其他微量元素的影响.方法 将48只3周龄雄性SD大鼠随机分为对照组、膳食纤维组、单纯染锰组(50 mg/kg)、染锰+高、中、低剂量膳食纤维组(分别在饲料中参入16%,8%和4%的膳食纤维);1个月后处死大鼠,测定大鼠体重、24 h粪锰以及脑、肝组织和血液中锰、铜、铁、锌含量.结果 染锰+高、中剂量膳食纤维组24 h粪锰排出率分别为29.44%,28.49%高于单纯染锰组的20.87%(P<0.05);染锰+高、中、低剂量膳食纤维组脑、锰含量分别为1.37,1.62,2.24,2.57μg/g肝组织和锰含量分别为1.78,2.73,2.54,2.70μg/g,明显低于单纯染锰组(P<0.01);脑、肝组织和血液中Cu、Fe、Zn含量在各组之间差异均无统计学意义(P>0.05).结论 膳食纤维能通过增加锰的排出以减少锰的蓄积.

Effects of dietary fiber on elimination of manganese in rats

Objective To explore the effect of dietary fiber on the elimination of manganese and other trace elements.Methods Forty eight three week old male SD rats were randomly divided into sixgroups:control group(group A),group with supplement dietary fiber in diet(group B),Mn treatment group(group C),group with treatment of Mn⁺ 16% fiber in feed(group D),group of Mn⁺ 8% fiber in feed(group E),and group of Mn⁺ 4% fiber in feed(group F).The ratswere sacrificed after one month treatment.The change of weight,24 hour manganese content in stool,Mn,Cu,Fe,and Zn contents in brain,liver tissue and blood were determined.Results Compared with the group C(20.87%),24 hour discharge ratios of manganese of in stool of group D and E(29.44%,28.49%)were increased(P<0.05).Mn contents in bra in and liver tissue and blood in groug A(1.37 μg/g,1.78 μg/g,0.24 μg/ml),group D(1.62 μg/g,2.73 μg/g,0.35 μg/m l),group E(2.24 μg/g,2.54 μg/g,0.37 μg/m l),and group F(2.57 μg/g,2.70 μg/g,0.38 μg/ml)were decreased compared to group C(3.59 μg/g,3.22 μg/g,0.48 μg/ml; P<0.01).Cu,Fe and Zn contents in the of brain,liver tissues and blood of the rats had no significant difference among all groups(P>0.05).Compared with group A(35.99%),the change of weight ratio was decreased in groups C and F(25.98%,27.03%;P<0.05).Conclusion Die tary fiber may decrease manganese accumulation by increasing the discharge of Mn in stool.