六磷酸肌醇对人胰腺癌细胞株PC-3和人胰腺原代培养细胞增殖分化的影响

目的观察六磷酸肌醇(IP6)在不同浓度、不同时间作用下对胰腺癌细胞株PC-3和正常胰腺原代培养细胞生长、分化的影响。方法直接细胞记数绘制生长抑制曲线、MTT法、流式细胞计检测细胞凋亡等方法观察PC-3细胞株和原代培养细胞增殖、分化特性。结果IP6对PC-3胰腺癌细胞的抑制呈时间、剂量依赖性,10 mmol/L时抑制作用达到最强,5mmol/L时的抑制效果稍小于10 mmol/L;IP6在<5 mmol/L时对人胰腺原代培养细胞的增殖几乎无影响,5 mmol/L时有轻度的抑制作用,10 mmol/L有明显的抑制作用。结论5 mmol/L的IP6既最大限度的杀伤肿瘤细胞,又最小程度的干扰非靶细胞,为最佳抑癌浓度。

The Influence of inositol hexaphosphate on proliferation and differentiation of human pancreatic adenocarcinoma cell line PC- 3 and human pancreatic primary culture cell

Objective To observe the effects of IP6 on human pancreatic adenocarcinoma cell line PC-3 and human pancreatic primary culture cell proliferation and differentiation in vitro.Methods Direct counting cells for cell growth curve,MTT test,flow cytometry test cell apoptosis were used to observe cell proliferation and differentiation.Results IP6 inhibited the growth of human pancreatic adenocarcinoma cell line PC-3 in a time and dose dependent manner.The effect was biggest when the level was 10mmol/L,but little difference with 5mmol/L and 10mmol/L.When less than 5mmol/L,IP6 almost can not effect the growth of human pancreatic primary culture cell;only little inhibition in 5mmol/L,and it was obviously inhibited in 10mmol/L.Conclusion When 5mmol/L,IP6 is the best inhibited density,which could kill tumor cell utmost and interfere normal cell in the lowest level.