远志不同炮制品的急性毒性比较研究

目的比较生远志、制远志、蜜远志60%乙醇提取液及水提取液对小鼠的急性毒性,为远志的毒性研究及临床安全用药提供科学依据。方法分别制备生远志、制远志、蜜远志的60%乙醇提取液及水提取液,测定其总皂苷含量,并采用灌胃给药,观察14天内小鼠的中毒情况及死亡情况,用改良寇氏法分别测定其半数致死量及95%可信限。结果制远志乙醇提取液中总皂苷含量最高,其毒性最大;而蜜远志水提取液中的总皂苷含量最低,其毒性最低。结论不同炮制方法能改变远志皂苷类成分的含量,远志的毒性与总皂苷的含量成正比,总皂苷含量越高,其毒性越大。

Study on Acute Toxicity Test of Different Processed Products of Radix Polygalae

Objective To study the acute toxicity of the water extracts(ERWE) and 60% ethanol extracts(EREE) from different processed products of Radix Polygalae(crude Radix Polygalae,licorice,and honey processed Radix Polygalae) ,thus providing scientific evidence for toxicity study of Radix Polygalae and its safe clinical application.Methods The ERWE and EREE were prepared from different processed products of Radix Polygalae.Their contents of saponins were respectively determined.The poisoning condition and death of the mice administered with ERWE and EREE by gastrogavage were observed within fourteen days.The modified Karber’s method was used to calculate LD50 and 95% confidence interval(CI) .Results The EREE of licorice processed Radix Polygalae had the maximum toxicity with highest content of saponins,while the ERWE of honey processed Radix Polygalae had the minimum toxicity with lowest content of saponins.Conclusions Different processing methods have effects on the contents of saponins in Radix Polygalae.The experiment showed that the toxicity of Radix Polygalae is in direct proportion to the content of saponins.The higher the saponins contents,the higher the toxicity.