滋补脾阴方药对脾阴虚糖尿病大鼠海马胰岛素抵抗影响的实验研究

目的探讨滋补脾阴方药对脾阴虚糖尿病大鼠海马胰岛素信号通路改变的保护作用及其可能的机制。方法高脂饮食加注射小剂量链脲佐菌素(STZ)建立2型糖尿病模型。脾阴虚模型参照经典的复合因素造模法建立。行为学测试大鼠学习记忆能力。Western blot检测海马IRE1α、JNK、IRS-1蛋白表达情况。结果脾阴虚糖尿病大鼠学习记忆能力与空白对照组比较,差异有统计学意义(P<0.05)。滋补脾阴方药治疗后学习记忆能力改善。脾阴虚糖尿病大鼠海马中磷酸化IRS-1、JNK及IRE1α蛋白表达增强(P<0.05),滋补脾阴方药治疗后表达减弱。结论滋补脾阴方药可显著改善脾阴虚糖尿病大鼠学习记忆障碍。其作用可能与改善内质网应激来调节胰岛素信号通路有关。

Effects of Zibu Piyin Recipe on the Insulin Resistance in the Hippocampus of Pi-Yin Deficiency Diabetic Rats

Objective To study the protection of Zibu Piyin Recipe(ZBPYR) on the insulin signal pathway in the hippocampus of Pi-yin deficiency diabetic encephalopathy rats and to explore its possible mechanisms.Methods The type 2 diabetic model was established using high fat diet and intraperitoneal injection of small dose streptozotocin(STZ) .The Pi-yin deficiency model was established referring to classic compound factors.The learning and memory capabilities were tested in rats by the behavioral changes.The protein expressions of hippocampal IRE1α,JNK,and IRS-1 were detected using Western blot.Results There was statistical difference in the learning and memory capabilities of Pi-yin deficiency rats when compared with the blank control group(P<0.05) .The learning and memory capabilities could be improved by ZBPYR.The protein expressions of hippocampal phospho-IRS-1,phospho-JNK,and total IRE1α were enhanced(P<0.05) .But they were weakened after treatment of ZBPYR.Conclusions ZBPYR could significantly improve the learning and memory capabilities of Pi-yin deficiency diabetic rats.Its functions might be correlated with improving the endoplasmic reticulum stress to regulate the insulin signaling pathway.