乙型肝炎病毒DNA含量与血清标志物的关系

目的 探讨乙型肝炎患者血清乙型肝炎病毒（HBV）DNA定量与乙肝血清标志物关系。方法 采用定量聚合酶链反应（PCR）法检测354例乙型肝炎患者血清中DNA含量，与HBV标志物作对比研究。结果 血清HBeAg阳性组HBVDNA含理明显高于抗-HBe或抗-HBc阳性组，而后两组HBVDNA检出率仍较高，乙型肝炎肝硬化组阳性率明显低于慢性乙型肝炎组，且含量较低。结论 定量PCR法检测HBVDNA检出率仍较高，乙型肝炎肝硬化组阳性率明显低于慢性乙型肝炎组，且含量较低。结论 定量PCR法检测HBV DNA具有较强的特异性和灵敏度，为临床了解病毒复制情况，制定治疗方案及疗效观察提供了确切依据。

The relationship between the amount of HBV DNA in serum and HBV markers.

Objective To study the relationship between the amount of HBV DNA in serum of patients with hepatitis B and the HBV serological markers. Methods The amount of HBV DNA in the serum of 354 patients with hepatitis B was detected with quantitative PCR. At the same time, we also detected the serum HBV markers of these patients with ELISA. Results The concentration of HBV DNA in HBeAg positive group was obviously higher than that in anti HBe or anti HBc positive group. But the positive rate of HBV DNA was still high in the two later groups. The HBV DNA positive rate of cirrhosis group was much lower than that of chronic hepatitis group, and the concentration in the cirrhosis group was also very low. Conclusion Quantitative PCR, which we use to detect the serum HBV DNA, was very specific and sensitive. It provided definite clinical basis for studying the duplicative state of the hepatitis B virus, making therapeutic plans and observing the effect of therapy.