大面积烧伤患者早期红细胞膜胆固醇变化与红细胞变形性的关系

目的研究大面积烧伤患者早期红细胞膜胆固醇与红细胞变形指数的变化及意义。方法利用核孔滤膜法测量红细胞变形指数 ,使用化学修饰电极测量红细胞膜胆固醇。结果大面积烧伤患者早期与正常对照组比较红细胞变形指数显著下降 ,分别为 7.82 6± 0 .5 3及 6 .5 41± 0 .6 1,而红细胞膜胆固醇则显著升高 ,分别为(0 .6 45± 0 .0 6 2 ) mm ol/ g膜蛋白及 (0 .891± 0 .0 5 1) m mol/ g膜蛋白 (P<0 .0 1) ,且二者呈高度负相关 (r=- 0 .80 1)。结论红细胞膜胆固醇能够有效反映大面积烧伤患者早期红细胞变形能力 ,并成为反映大面积烧伤的早期微循环状态的新型量化指标

The relationship of erythrocyte membrane cholesterol and erythrocyte deformability in patients with great area burn

Objective To study the significance of cholesterol levels on erythrocyte membrane and erythrocyte deformability in patient with great area burn. Method The cholesterol level on erythrocyte membrane and the index of erythrocyte deformability were measured by methods of chemically modified electrode and percolation respectively. Results The amount of cholesterol on erythrocyte membrane in patients with great area burn was higher than that in normal controls and the index of erythrocyte deformability in patients with great area burn was lower than that in normal controls. There was highly negative correlation between patients and controls (r=-0 801). Conclusion Erythrocyte membrane cholestrol may be used as a new index for erythrocyte deformability and the state of microcirculation.