慢性阻叁性肺疾病急性加重期患者血清基质金属蛋白酶9浓度与肺通气功能变化的关系

摘要目的探讨慢性阻塞性肺疾病急性加重期（AECOPD）患者的血清MMP-9、TIMP-1浓度的变化及其与肺通气功能变化的关系。方法采用酶联免疫吸附（ELISA）方法检测56例AECOPD患者和32名健康人血清中MMP-9、1 TIMP-1浓度。结果AECOPD患者血清MMP-9、TIMP-1浓度（126．89±113．84）ng／mL、（226．28±105．133）ng／mL]明显高于对照组i（28．65±16．43）ng／mL、（132．69±40．41）ng／mL，P〈0．01I。AECOPD组和对照组血清MMP-9、r11MP-1浓度与FEV1占预计值％具有负相关性（r=-0．591、-O．651，P〈0．01）；AECOPD组和对照组血清MMP-9、TIMP-1浓度与FEV1／FVC％具有负相关性（r=-0．558、-0．653，P〈0，01）；MMP-9／TIMP-1比率与FEV1占预计值％及FEV1／nrC％具有负相关性（r：-0．363、-0．356，P〈0．05）。结论MMP-9、TIMP-1是引起肺通气功能下降很重要的因素。

Relation Ship of Matrix Metalloproteinase- 9 and Tissueinhibitor of Matrix Metalloproteinase - 1 with Pulmonary Function in Patients with Chronic Obstructive Pulmonary Disease

Objective To investigate the change of matrix metallop roteinase（MMP） - 9 ,tissue inhibitor of matrixmetallop rotein- ase（TIMP） -1 expression in the serum of patients with AECOPD as well as the correlation between the expressions of these factors and lung function. Methods The levels of serum MMP - 9 and TIMP - 1 in 56 patients with AECOPD and 30 healthy donors were ex- amined by enzyme - linked immunosorbent assay （ EL ISA）. Results The serum levels of MMP - 9, TIMP - 1 （ 126.89 ＋ 113.84） ng/ mL, （226.28 ＋ 105. 133）ng/mL] in the patientswith COPD were statistically significant higher than those of the control group （28. 65 _＋ 16.43 ） ng/mL, （ 132.69 --. 40.41 ） ng/mL,P 〈 0.01 ]. There was Significantly negative correlation between the levels of MMP - 9, TIMP - 1 in serum and FEV1% （ r = - 0.591, - 0.651, respectively ,P 〈 0.01 ）. This negative correlation was also seen between MMP - 9,TIMP - 1 and FEV1/FVC% （ r = - 0. 558, - 0. 653, respectively, P 〈 0.01 ）. Further - more, negative relationship swere observed between the ratio ofMMP -9/TIMP - 1 and FEV1%, as well as MMP- 9/TIMP- 1 and FEV1/FVC% （r = -0.363, -0. 356 respectively ,P 〈 0.05 ）. Conclusion MMP- 9 and TIMP- 1 may play an important role in the development of airway obstruction.